Study On Isolation, Purification And Function Of Liquid Fermented Nonferrous Products From Bamboo And Yellow Separation

Shiraia bambusicola is a kind of precious traditional Chinese medicine, which contains a variety of stroma active ingredients, such as hypocrellin, Shiraia bambusicola polysaccharide, with menstruation scattered stasis, cough expectorant, physical fitness, and sedative effects. With unceasingly carries on the thorough research, the medicinal value of Shiraia bambusicola is more and more prominent, but because of the limited natural Shiraia bambusicola resources to meet the needs of its people, people began to study Shiraia bambusicola separation strains, looking forward to get Shiraia bambusicola active ingredient. In this paper, while Shiraia bambusicola isolate-Arthrinium arundinis act as the tester strain, we mainly studied its growth law, separation and identification of the pigment, the stability of the pigment and functional tests of the raw pigment.results are as follows:1. The growth law of Shiraia bambusicola separation strains:In the solid medium, primary mycelium is white, radiating grow to the surrounding. Four days later, it began to produce red pigment, which is present in the culture medium. In the later, mycelium color became darkened, with slow growth; In the liquid medium, the mycelium is white, sticky and smooth, and scattered. Three days later, it began to produce red pigment, which is present in the culture medium. In the later, it grew slowly.2. Separation and identification of the pigment:As using macroporous adsorption resin to isolate pigment, we select HPD300 as adsorption resins. While 50% ethanol was eluent, we got the enrichment of pigment.With thin layer chromatography and high performance liquid chromatography, we found that the polarity of pigment was very strong, and it was difficult to achieve the effective separation of the pigment with conventional method. With the fermentation liquid of acid hydrolysis, organic solvent extraction and two silica gel column chromatography, we got two kinds of high purity substances.Through mass spectrometry, molecular weights of two substances were 191 and 338. By colorless methylene blue color reaction, the anthraquinone is generally able to be denied and it is likely to be benzoquinone or naphthoquinone.3. The stability of pigment:By the study of the stability of pigment produced by Shiraia bambusicola separation strains, temperature and light have a little impact on the stability of pigment, while the stability of the pigment is affected by pH and metal ions obviously. It was more stable in an acidic environment than in an alkaline environment. The different metal ions have different impacts on the stability of pigment. In a certain concentration range, Mg2+, Ca2+, Zn2+, Al3+, Cr3+, Cu2+, Na+have a little impact on the stability of pigment, and Fe3+has subtractive effect.4. Functional tests of the raw pigment:Through antibacterial test of red pigment produced by Shiraia bambusicola separation strains, we found that the pigment can strongly inhibit the growth of Fusarium solani and Aspergillus niger, while it has a little impact on Bacillus subtilis and Staphylococcus. Through anti-oxidation test, we found that the antioxidant capacity of the pigment is not very strong.