| Toluene is widely used in industrial process, and its chemical property is very stable. Because of its bioaccumulation, toxicity and carcinogenicity, it was listed as priority pollutants by EPA. To control the discharge of such pollutants, biodegradation technology has several advantages such as high-efficiency, low-energy consumption, ambient reaction conditions, and low secondary pollution.A fungal strain was obtained in this work. It was assigned to be Trichoderma viride, and named as LW-1. It was possibly to be a new species which was able to degrade toluene. In the flask test, although there are certain fungi LW-1 of toluene degradation, the degradation efficiency is low and cannot be degraded completely. The optimal conditions for the growth of LW-1 were at 30 and pH 4.8. The diversity of biodegradation ℃was also tested in the study, it was found that the strain LW-1 had high degradation efficiency of ethyl acetate.Being more adaptive to a low humidity, the high humidity may not be conducive to the growth and reproduction of fungi in the flask test. Therefore, the strain LW-1 was inoculated into the biofilter reactor in order to strengthen the degradation of waste gas with toluene contained. To research the strengthening of the strains, fungi only and bacteria + fungi were inoculated in two biofilter reactor, respectively. Within 18 days of operation, two BF’s start-up basically completed. A shorter start-up time and better performances on the removal of toluene were attained in the bacteria + fungi biofilter reactor. It got the best result on the removal rate, the maximum removal load and the CO2 production loads under different operating conditions. It indicated “the mode of inoculation fungal and bacterial” has a high removal performance, and the combination of different strains has a significant strengthening effect. The combination of fungi and bacteria has obvious advantages in the filter column under high concentration waste gas compared with the fungi- only filter.In this paper, by using real-time fluorescence quantitative PCR and high-throughput sequencing and other molecular biological methods, the two different sets of biological filtration tower’s biological membrane, biological population structure, diversity and dynamic rules were studied. Real time fluorescence quantitative PCR detection showed that the number of bacteria in start-up period. The F-BF tower of the bacteria was significantly less than the F&B-BF tower, the F-BF tower because of streptomycin sulfate was added in the nutrient solution to inhibited the growth of bacteria. It showed no differences in the population of archaea. However double population of fungi was detected in F-BF than F&B-BF. In the stable operation stage, the population of bacteria in F&B-BF tower was improved evidently, the population of fungi was significantly higher with an order of magnitude in F&B-BF tower than that of F-BF. The structures of archaea were still the same at this period of time. High throughput sequencing results showed that if the 817F/1196 R was primer, Zygomycota, Hypocreales, Saccharomycetales, Eurotiales, Conthreep and some other strains were dominant in the biofilter tower with the inoculation of fungi only. In the biofilter inoculated with both fungi and bacteria, Chaetothyriales, Tremellales, Hypocreales, Eurotiales and some other strains were dominant. If the 338F/806 R was primer, Burkholderiales 、 Xanthomonadales 、 Lactobacillales and some other strains were dominant in the biofilter with the inoculation of fungi only. In the biofilter inoculated with both fungi and bacteria, Burkholderiales、Corynebacteriales、Rhodospirillales、Xanthomonadales and some other strains were dominant. |
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