| On the basis of analysis about the physicochemical properties and glyceride content of peony seed oil and fatty acid composition, triglycerides structure and sn-2 distribution of fatty acid and lipid content composition of the peony seed oil, the enzymatic esterification deacidification, high vacuum molecular distillation refining method and technology of the deacidification condition were discussed emphatically of the peony seed oil of high acid value.Researched by using classical and modern instrumental analysis methods, determination of the physical and chemical characteristics of peony seed oil, the results showed that the iodine value, saponification value, and the proportion of the physical and chemical indexes accord with the requirement of national edible oil; The fatty acid compositions were analyzed by using gas chromatography,the relatively content of unsaturated fatty acids was more than 91.96%,the content of the oleic acid was 22.83% ~ 22.83%, the content of the linoleic acid was 25.43%~27.51%,linolenic, the content of linolenic acid was up to 40.50%~44.34%; Glyceridecontent by thin layer chromatography-flame ionization detector of the peony seed oil, the content of the monoglycerides monoglycerides was 0.27% to 0.29%, the diacylglycerols was 3.31% to 4.29%, while the triglycerides was above 95%; By the method of pancreas lipase hydrolysis, the sn-2 position fatty acids were analyzed and the triglycerides composition of peony seed oil were calculated according to 1,3-random-2-random distribution doctrine. The mainly fatty acids were oleic acid, linoleic acid and linolenic acids,total content as high as 97.28%, Among them, the content of oleic acid was 42.01%, linoleic acid was 32.23, linolenic acid was 23.04%, the saturated fatty acid was only 3.72%; The content of three unsaturated fatty acid esters was 71.00%,and a saturated two unsaturated fatty acid ester content was more than 15.00%; The vitamin E content of the peony oil was 41.61 mg/100 g to 56.53 mg/100 gwhich was analyzed by using HPLC. Through Rancimat determination,the result of oxidation stability of the peony seed oil was weak, susceptible to oxidation and difficult to long-term storage.Research discusses the acid value of peony seed oil of refined acid method and the process conditions, The chemical alkali refining deacidification, the acid value of peony seed oil was decreased from the original 9.87 mg/g to 0.22 mg/g, the acid value of the deodorization peony seed oil ultimately dropped to 0.21 mg/g, trans fatty acid content was 1.87%. Water vapor physical deacidification method in reaction time 10 h, temperature of 200 ℃, 220 ℃ and 240 ℃, finally acid value were only 8.17 mg/g, 3.90 mg/g and 1.57 mg/g, the deacidification temperature is 240 ℃, the deacidification time was from 2h to 10 h, total content of trans fatty acids increase quickly, increased from 0.40% to 20.01%, well above the national recommendation was less than 1%.The high vacuum molecular distillation refining method and technology of the deacidification condition were discussed emphatically of the peony seed oil of high acid value. The results showed that the deacidification technology of peony seed oil molecular distillation was studied, the optimal process parameters as follows: the reaction temperature 190 ℃,the blow film speed was 235r/min, the The blanking speed was 2 ml/min. Under the conditions,the the acid value of the penoy oil was 1.21 mg/g.The results showed that the optimum process parameters:the reaction time was 10 h, the reaction temperature 65 ℃,the enzyme dosage of the Lipozyme RMIM was 4%, the dosage of the glycerol was 200%. Under the conditions,the the acid value of the penoy oil was decreased from 18.55 mg/g to 2.42 mg/g. |
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