Study On The Spectral Characteristics Of Interaction Between Several Commonly Used Pesticides And Serum

Pesticide residues and human health has become an important issue to let humanity concern.So this paper through spectroscopic technique studied the effect relationship between severalcommonly used pesticides (tebuconazole, paraquat, phoxim, propineb, pyrazosulfuron ethyl,avermectin chlorpyrifos) and human serum with the specific conditions of the laboratory, theresults provide a reference for pesticide poisoning emergency medical. The main research workincludes the following aspects:(1) By UV-visible absorption spectroscopy, fluorescence spectroscopy technique to studythe spectral characteristics of several commonly used pesticides. By UV Vis absorptionspectroscopy is obtained for the different pesticides in the UV visible region have respectivecharacteristic absorption peak, their peak number and peak shape are not the same; Byfluorescence spectroscopic techniques is obtained for at different excitation wavelengths,different pesticides have different fluorescence regions, the maximum fluorescence peak aredifferent, corresponding to the optimal excitation wavelength is not the same. The results can beanalyzed for pesticides qualitative reference.(2) Using UV-visible absorption spectroscopy to study the spectral characteristics of theinteraction between pesticides and serum (high-sugar, high-fat), and the different concentrationsof pesticides effects on serum. Through study, positive, abnormal serum absorption peaks at278nm when added pesticides, which Weakening of the serum absorbance strength. Differentconcentrations have an offset phenomenon for pesticide absorption peak serum. By experimentalanalysis obtained the relational model between the values of the pesticide concentration and theabsorbance. This model can be quantitatively analyzed for pesticide residues in blood samples.(3) By fluorescence spectroscopy to study the spectral characteristics of pesticides and therole of serum. By studying the effect of serum obtained before and after pesticide generated, serabest excitation wavelength290nm, the peak position is336nm. Tebuconazole adding serummakes the maximum fluorescence peak red shift3nm (336to339nm), paraquat added serummakes the optimum excitation wavelength red shift to295nm. At the same time, the fluorescenceintensity of human serum was decreased obviously by the addition of pesticides. Results forpesticide residues in blood provide evidence for qualitative analysis. (4) Based on the synchronous fluorescence spectra and fluorescence spectra of three–dimensional effects of pesticides and other technologies for the serum study. At the wavelengthdifference of60nm,70nm,80nm, the fluorescence spectra of the pesticides the impact is obvious,the peak red shift or blue shifted bigger. Under the constant wavelength difference15nm and60nm, the pesticide has an effect on the tyrosine residues and tryptophan residues in the serum.Through the three-dimensional fluorescence spectrum technology is obtained for serum has twofluorescence peaks, the peak value was334nm and336nm, and the corresponding excitationwavelength was260nm and290nm. The fluorescence intensity of the pesticide after addingpesticide in serum was quenched, and the peak position and the corresponding excitationwavelength were changed.