The Study Of Quality Control And Activity Evaluation For Tartary Buckwheat Fermented Wine

As the “king of the grains”. Tartary buckwheat is a medical and edible minor grain crop, not only rich in amino acids, fatty acids(VF), dietary fiber, carbohydrates and minerals but also rich in high active functional ingredients as biological flavonoids, gamma-aminobutyric acid and D-chiro inositol, which give Tartary buckwheat special health and physiological function. Yellow wine is one of the oldest wine in the world, enjoy the reputation of “national wine”, with beer, grape wine are the world’s three largest ancient wine. Yellow wine comes from China and exists in China only.Using rice, millet, wheat as the main raw materials, with the processes of cooking, cooling, adding koji, falling cylinder nest and saccharifying fermentation can we get this brewing wine. The optimum fermentation condition: koji dosage 0.11%, starter dosage 0.26%, adding alcohol 25%vol, alcohol degrees:raw material quantity is 1:1, fermentation temperature 30℃, a rake every three days, sealing for fermentation after 2~3 times.We studied the quality control and evaluation of activity of the Tartary buckwheat fermented wine( TBFW). The main results are as follows:1. Quality control of Tartary buckwheat fermented wine1.1 To establish a RP-HPLC method for simultaneous determination of polyphenols in TBFW, 6 kinds of polyphenols, rutin, coumaric acid, different quercetin, ferulic acid, quercetin, kaempfrol were detected in TBFW. The chromatographic conditions: 0.2% phosphoric acid water(A phase), acetonitrile(B phase), 0 to 10 min, 18%B; 10 to 35 min, 45%B; 35 to 40 min, 75%B; 40 to 42 min, 98%B. Flow rate: 1.0mL/min; wavelength: 360nm; column temperature: 30℃; inject volume: 20μL. The determination results showed that rutin was the most abundant polyphenols in Tartary buckwheat fermented wine and its content up to 302.25μg/mL, followed by quercetin: 36.20μg/mL.1.2 To establish a RP-HPLC-ELSD method for determination D chiro inositol(DCI) in TBFW, The chromatographic conditions: water(A phase), acetonitrile(B phase), 0 to 20 min, 80%B; 20 to 50 min, 35%B; Flow rate: 1.0mL/min; Evaporation temperature: 30℃; nebulization temperature: 90℃; Inject volume: 20μL. The measured content of DCI was 91.48 μg/mL.1.3 The establishment of the fingerprint for 10 batches of TBFW by using RP-HPLC method. The results showed that 10 batches of samples with high similarity, there was no significant difference between all batches, this method can control the quality of TBFW to some extent, in case of TBFW adulteration.1.4 Using the method of GC-MS to analysis the volatile components in the fermentation process of TBFW. The results showed that: aldehyde matter was consumed and its content decreased in the later fermentation process; alcohol increased; the content of total acids increased firstly, then decreased, L-lactic acid decreased with the fermentation process; with the fermentation process continuing, the esterification reaction was occurred, ester types and content increased constantly.1.5 To determine and analysis the physical and chemical indexes in TBFW’s later fermentation process. Total acid, amino nitrogen, total sugar, pH and alcohol were detected. Each index trends to be stable and within the the scope of GB/T13662-2008 regulation.2. Activity evaluation of Tartary buckwheat fermented wine2.1 Antioxidant activityWe studied the effect of TBFW on experimental D-galactose induced oxidative stress in aging mice, the results showed that after intake of the TBFW, the 8 iso prostaglandin(8-iso-PG) decreased in serum((p< 0.05)), lipid peroxidation product MDA and protein carbonyls were significantly decreased in brain and liver(p< 0.01). In addition, the activity of SOD and GSH-px increased significantly in brain and liver(p< 0.01). So TBFW has certain antioxidant activity, especially has significant influence on damaged liver and brain induced by oxidation, and can be used to repair the brain and liver tissue.2.2 The research of anti-fatigue effectsTo study the anti-fatigue effects on mice. TBFW can shorten the opening rest time, prolong moving time and moving distance, the influence of medium and high dosages were significant(p <0.01,p <0.05); In addition, TBFW can prolong the swimming time, improve the endurance, medium dosage is significant(p <0.05). So TBFW has certain anti-fatigue function.