Electrochemical Biosensors Based On Ordered Mesoporous Carbon/conducting Polymers

Taking advantage of large specific surface area, order pore structure, superior thermodynamic stability, conductivity of ordered mesoporous carbon(OMC) and excellent conductivity, special spatial structure, having active radical in high concentration, stable electrochemical properties of conducting polymer, novel electrochemical biosensors based on OMC/conducting polymer were developed. Those biosensors were used for determination of dopamine and uric acid. The main contains are as follows:(1) A glassy carbon electrode was modified by acid-treated ordered mesoporous carbon(f-OMC) combined with the ionic liquid(IL). Then the biosensor based on f-OMC/IL-PST was fabricated by electrochemical polymerization of Safranine T(ST) on the surface of the electrode through cyclic voltammetry. The electrocatalytic performance and mechanism of the sensor toward dopamine and uric acid were discussed. The sensing properties of the sensor to dopamine and uric acid were studied by differential pulse voltammetry(DPV). Furthermore, we demonstrate successfully its application for dopamine and uric acid detection in human blood serum and dopamine detection in dopamine hydrochloride injection.(2) OMC-MB dispersion was prepared by ultrasonication though the π-π interaction between OMC and methylene blue(MB). And an dopamine sensor based on OMC-PMB/CS(chitosan) was prepared by the dropping dispersion and electro-polymerization method. The formation mechanism of the polymer and the electrochemical behavior of dopamine on the surface of the modified electrode were studied. And also the sensing properties were discussed. Further, the practical samples were tested.(3) OMC-MB composites were synthesised by ultrasonication. A sensor based on the OMC-PMB/IL was prepared by rubbing the composites and ionic liquid and subsequent electro-polymerization method to modify the glass barbon electrode. The electrocatalytic performance and sensing properties of the sensor toward dopamine were discussed. At the same time the sensor was used in the detection of real samples.