Study On The Influence Of Antibiotics On Mariculture Wastewater By Sequencing Batch Biofilm Reactor

With the rapid development of China’s aquaculture industry, coastal farms and creating wastewater was a rapid rise, making the deterioration of the environment near the sea, resulting in the destruction of coastal ecosystems, ecological balance, frequent red tides and breeding and other diseases hazards. The impact of environmental pollution on the mariculture industry more and more attention by scholars, and has become one of the hot current farming ecological science.This paper uses sequencing batch biofilm reactor (SBBR) on aquaculture wastewater which contains chlortetracycline, florfenicol, sulfadiazine and norfloxacin. The research results of the research in this lesson,using anaerobic+aerobic (residence time 3:7) run way and evaluation of antibiotics on the influence of nitrification and denitrification process.This topic through the extracellular polymers (EPS) and activated sludge reaction rate (specific oxygen utilization rate (SOUR)、specific ammonium oxidation rate (SAOR)、specific nitrite oxidation rate (SNOR) and specific nitrate reduction rate (SNRR)), reveals the influence on the effect of antibiotics on mariculture wastewater treatment and its influence to the changes of microbial activity and EPS.With the increase of chlortetracycline concentration from 0 mg/L to 35 mg/L, the average removal efficiencies of COD and NH4+-N from 95% to 79% and from 96% to 82% in a SBBR, respectively. As the chlortetracycline concentration increased from 0 mg/L to 35 mg/L, SOUR of activated sludge decreased from 12.12mgO2/gMLSS/h to 4.54mgO2/gMLSS/h, and SAOR, SNOR and SNRR of activated sludge decreased from 1.57mgO2/gMLSS/h,1.21mgO2/gMLSS/h and 3.08mgO2/gMLSS/h to 0.84mgO2/gMLSS/h,0.61mgO2/gMLSS/h and 2.02mgO2/gMLSS/h, respectively. The PN/PS ratios in LB-EPS and TB-EPS increased from 1.31 and 1.24 to 1.91 and 2.69 with the increase of chlortetracycline concentration. There are five peaks identified in SMP, five peaks identified in LB-EPS and four peaks identified in TB-EPS by. 3D-EEM fluorescence spectroscopy. FTIR spectra suggested that the chlortetracycline concentration variation has distinct effects on the functional groups in SMP, LB-EPS and TB-EPS.With the increase of florfenicol concentration from 0 mg/L to 35 mg/L, the average removal efficiencies of COD and NH4+-N from 93% to 65% and from 95% to 78% in a SBBR, respectively. As the florfenicol concentration increased from 0 mg/L to 35 mg/L,SOUR of activated sludge decreased from 12.21mgO2/gMLSS/h to 2.71mgO2/gMLSS/h,and SAOR, SNOR and SNRR of activated sludge decreased from 1.49mgO2/gMLSS/h,1.17mgO2/gMLSS/h and 3.03mgO2/gMLSS/h to 0.61mgO2/gMLSS/h,0.48mgO2/gMLSS/h and 2.04mgO2/gMLSS/h, respectively. The PN/PS ratios in LB-EPS and TB-EPS increased from 1.45 and 1.23 to 1.91 and 2.75 with the increase of florfenicol concentration. There are five peaks identified in SMP, four peaks identified in LB-EPS and three peaks identified in TB-EPS by 3D-EEM fluorescence spectroscopy. FTIR spectra suggested that the florfenicol concentration variation has distinct effects on the functional groups in SMP, LB-EPS and TB-EPS.With the increase of sulfadiazine concentration from 0 mg/L to 35 mg/L, the average removal efficiencies of COD and NH4+-N from 93% to 76% and from 94% to 83% in a SBBR, respectively. As the sulfadiazine concentration increased from 0 mg/L to 35 mg/L,SOUR of activated sludge decreased from 11.71mgO2/gMLSS/h to 4.41mgO2/gMLSS/h,and SAOR, SNOR and SNRR of activated sludge decreased from 1.60mgO2/gMLSS/h,1.14mgO2/gMLSS/h and 2.97mgO2/gMLSS/h to 0.99mgO2/gMLSS/h,0.60mgO2/gMLSS/h and 1,29mgO2/gMLSS/h, respectively. The PN/PS ratios in LB-EPS and TB-EPS increased from 1.25 and 1.24 to 2.13 and 3.14 with the increase of sulfadiazine concentration.There are three peaks identified in SMP, four peaks identified in LB-EPS and three peaks identified in TB-EPS by 3D-EEM fluorescence spectroscopy. FTIR spectra suggested that the sulfadiazine concentration variation has distinct effects on the functional groups in SMP, LB-EPS and TB-EPS.With the increase of norfloxacin concentration from 0 mg/L to 35 mg/L, the average removal efficiencies of COD and MV-N from 92% to 79% and from 94% to 78% in a SBBR, respectively. As the norfloxacin concentration increased from 0 mg/L to 35 mg/L, SOUR of activated sludge decreased from 13.03mgO2/gMLSS/h to 4.35mgO2/gMLSS/h,and SAOR, SNOR and SNRR of activated sludge decreased from 1.49mgO2/gMLSS/h,1.18mgO2/gMLSS/h and 3.17mgO2/gMLSS/h to 0.81mgO2/gMLSS/h,0.25mgO2/gMLSS/h and 1.68mgO2/gMLSS/h, respectively. The PN/PS ratios in LB-EPS and TB-EPS increased from 1.26 and 1.18 to 2.02 and 2.94 with the increase of norfloxacin concentration. There are five peaks identified in SMP, four peaks identified in LB-EPS and five peaks identified in TB-EPS by 3D-EEM fluorescence spectroscopy. FTIR spectra suggested that the norfloxacin concentration variation has distinct effects on the functional groups in SMP, LB-EPS and TB-EPS.