Anti - Tumor Effect Of Curcumin And Its Derivatives On Breast Cancer MCF - 7 Cells And Its Mechanism

This article mainly studied the anti-tumor activity and anti-tumor mechanism of CUR, DMC, THC. The study tested the viability of tumor cells by MTT colorimetric method, and tested the cytotoxicity effect on tumor cell by LDH; DAPI was used to observa the cell morphology affected by CUR、DMC、THC. Flow cytometry was used to analyse the cell cycle and levels of reactive oxygen of MCF-7, then double staining was combined to analyse the apoptosis of cancer cells. According to the changes of the mitochondria transmembrane potential before and after apoptosis and the protein physicochemical properties which related to apoptosis, the anti-tumor mechanism of CUR、DM、THC was confirmed. The study found that the inhibitory action of tumor cell by CUR、DM、THC showed a time-dose dependent manner. And DMC had a stronger inhibitory action of tumor cell than CUR. The main results are as follows:(1) The results of anti-tumor experiment in vitro showed that CUR, DMC and THC had anti-tumor activity in a dose-dependent and time-dependent manner, Referring to the result of MTT and LDH, DMC, CUR and THC showed strong promoting effect on MCF-7 apoptosis, the IC50 was 101.3μM、112.4μM and 138.7μM, respectively. The flow cytometry was used to analyse the distribution of cell cycle and apoptosis, which showed that CUR、DMC、THC could induce cell cycle arrested in G0/G1 period and they all increased cellular apoptosis. More specifically, when MCF-7 cells were treated with 100μM of CUR、DMC、THC, there were 54.1%、55.8%、37.8% of the cell came into the early apoptosis. The research showed that the anti tumor effect of MCF-7 by CUR、DMC、THC were achieved through the cell cell cycle arrest and induction of cellular apoptosis, and DMC had the stronger antitumor effect than CUR.(2) DAPI staining method was used to determine the cells apoptosis of CUR、DMC and THC induced which can observed nuclear fragmentation and apoptotic body. This results proved that CUR% DMC and THC can increased cellular apoptosis.In addition, CUR、DMC and THC generated cell apoptosis via the mitochondrial pathway. The cytochrome C was released to cytosol and the loss of mitochondria membrane potential (△ψm) was observed after CUR、DMC and THC treatment. The results were evidenced by the activation of caspase-3 and caspase-9, the elevation of intracellular ROS, a decrease in Bcl-2 expression and an increase in Bax and PARP expression.