Preparation Of PLA Nanoparticles And Application Research As Immune Adjuvant

Nano technology is a new technology with development potential. Nanometer materials, with its small particle size, specific surface area, superior performance and other characteristics, has the extremely widespread application prospect. Preparation of biodegradable polymer nanoparticles in the biomedical industry has great research value.This thesis mainly studies the preparation of PLA nanoparticles and optimize the preparation process of nanoparticles, and the application of PLA nanoparticles as immune adjuvant research. Specific experimental results obtained as follows:1. We optimize the preparation technology of the nano-precipitation. Nano-precipitation’s process conditions are optimized by response surface method. Through the optimal conditions for preparation, PLA nanoparticles’ size is about 109.1 nm, and the PLA nanoparticles suspension has uniform particle size, good dispersibility;2. We study the methods of self emulsification solvent evaporation to prepare PLA nanoparticles, and optimize the method to best process conditions. The anionic surfactant SDS is applied to make negatively charged PLA nanoparticles. Through the optimal conditions for preparation, PLA nanoparticles’size is 126.8 nm, and PDI is 0.129, and Zeta potential is-53.7 mV, with uniformity and good dispersibility. And then we pick cationic surfactant DDAB as surfacant to make positive charge nanoparticles. Adopt the optimal conditions, the size of positively charged PLA nanoparticles is 101.7 nm, and its’PDI and Zeta potential are 0.078 and+37 mV respectively;3. We investigate the positively charged PLA nanoparticles as immune adjuvant’effects.Through animal immune experiments, we study the different size of PLA particles used as immune adjuvants,100 nm,300 nm and 1μm respectively. PLA nanoparticles as immune adjuvant’animal experiments prove that when the smaller PLA particles’size, the higher expression of antigen specific antibody in serum and cytokines, the better immune effect;4. We adopt three methods to make holes in negatively charged PLA nanoparticles, respectively by the methods of secondary emulsion swelling, carrier gas swelling and dynamic swelling method. We study the experimental process and device of three different methods. And experimental conditions are optimized by experiment. We succeed in making holes inside the nanoparticles.