Endophytic Bacteria Myriophyllum Spicatum Phosphate Solubilization Characteristics Of Its Host Plant Repair Of Water Bodies

Currently, eutrophication is the most serious pollution problems facing our natural waters. For the management of eutrophication have been used a variety of methods of physical, chemical, etc., but there are some drawbacks, eutrophication current bioremediation methods have greater advantages, application prospect, bioremediation plant, microbial remediation rich eutrophication of water bodies for emerging more effective treatmentmethods.Phytoremediation is an effect in recent years developed a good, low energy consumption, low cost of treating eutrophic water by new technology, but the north water plant growth cycle is relatively short, and the plants in the water and sediment and phosphorus absorption less, in order to overcome the above problems of eutrophication of aquatic plants in the restoration process encountered isolated from aquatic plant endophytes out joint action with aquatic plants and dissolved phosphorus have growth-promoting effects of eutrophication governance will is bioremediation technology has broad application prospects. In this study were isolated from ear Myriophyllum plants screened phosphate solubilizing bacteria in the role of four plants and by dissolving phosphorus ability to compare the experimental screening dissolved phosphorus in the value of having a high bacteria W3, discussed their Growth optimum culture conditions and dissolving characteristics under different culture conditions and endogenous soluble phosphorus strain W3 of growth-promoting potential application simulation, the role of sediment in the release of phosphorus forms and their host plants flower spike Myriophyllum repair promoting water bodies do a preliminary study. The main findings are as follows:Separated from the spike Myriophyllum out several strains of endophytes and dissolved phosphorus as a preliminary screening test within four plants have phosphorus solubilizing bacteria action, within four strains of bacteria make several soluble phosphorus feature comparison test, They were measured in four strains of bacteria cultured in phosphate solubilization medium 48 h, 96 h, 144 h supernatant concentrations of orthophosphate, to dissolve phosphorus was better strain W3, for example, three times the sampling time measured concentrations of orthophosphate as 56.16,269.60,282.05 mg / L(control group were 5.98,5.62,6.05 mg / L), due to the increased amount of 96 h 213.44 mg / L was significantly higher than 144 h increase the amount of 12.45 mg / L, the results show that achieve a better solution 96 h Phosphorus effect; of different phosphates(lecithin, aluminum phosphate, calcium phosphate) on the effect of phosphorus- solubilizing strains, the results show that the four strains best dissolve the calcium phosphate, orthophosphate concentration in the control group and the experimental group were added bacteria as 25.62,157.07,158.79,239.60,58.84 mg / L, dissolved phosphorus strains screened advantage W3; strains dissolved phosphorus results were obtained experimental studies within four strains of bacteria in different carbon and nitrogen source with glucose as the carbon source, ammonium sulfate as nitrogen source best dissolved phosphorus, W3 phosphate solubilization capacity of the strongest, the orthophosphate concentration 499.09 mg / L.Studies have shown that the strain W3 optimum growth pH range of 5.0 to 9.0, change the basal medium inorganic phosphorus carbon-nitrogen ratio of phosphorus- solubilizing strains W3 influential effect, strain W3 7.0 when dissolved phosphorus in the medium initial pH value works best good dissolution, fermentation pH values were significantly related to changes in calcium, high strain W3 indole acetic acid and siderophore production, has the potential to promote plant growth must, and the different forms of phosphorus in sediment of a certain release effect. Endogenous Phosphorous Bacteria W3 promote transformation of the role of phosphorus in the sediment and in the promotion of the host plant Myriophyllum Growth improve the host plant repair effect on the water body.