Preparation Of Basidiomycetes Fermentation Of Rice Bran Polysaccharide And Its Bioactivity

Rice Bran Polysaccharide is a function of factors has many physiological activity, mainly in the form of hemicellulose embedded in rice Ying peel. Because rice bran polysaccharide has a high added value, so the extraction and preparation of rice bran polysaccharide possesses good prospects for development.With traditional acid solution, ultrasonic, radiation, microwave digestion and the use of physical methods, such as small molecular weight of rice bran polysaccharide into fragments,compared with basidiomycete fungi extracellular enzymes from the fermentation of rice bran polysaccharide can greatly reduce the cost, and more environmental protection, and fungi in the production of extracellular enzymes at the same time, also secrete extracellular polysaccharide,fungal polysaccharide itself has a variety of immune activity and has the very high medicinal value, this experiment ferment rice bran polysaccharide by four strains belong to basidiomycotina.The research, polysaccharide content by phenol-sulfuric acid method to detect,monosaccharide composition and changes in sugar nitrile acetylation by gas chromatographic analysis to detect, sample molecular weight and distribution of the polysaccharide by eighteen-angle laser light scattering detection, rice bran polysaccharide sample oxidation resistance the strength of the effect by scavenging DPPH? inspection, testing the strength of rice bran sample oxidation resistance, through some samples of rice bran polysaccharide ability to stimulate the production of NO was measured macrophages concluded: ① When rice bran polysaccharide samples arabinose, xylose and galactose and mannose account for a large proportion, its oxidation resistance stronger. ② The molecular weight of the stronger immune activityrice of rice bran polysaccharide is limitde from 100 to 9999. ③ Combination of antioxidant activity analysis, the use of quasi Phellinus pine raw fermented rice bran polysaccharide, compared Schizophyllum, hair versicolor and sulfur bacteria is better. 7 to 9 days to get to the polysaccharide sample cleared DPPH? better, the yields of polysaccharide are1.1% to1.32%. In 9 days, the immune activity of polysaccharide samples were significantly higher than those before fermentating, the molecular weight of rice bran polysaccharide in 1000 ~ 9999 is highly active. ④ Through orthogonal experiment,the prescription of rice bran polysaccharide effervescent tablets Identified as rice bran polysaccharide 10 g, lactose 20 g, citric acid and tartaric acid totaling 15 g, PEG6000 with 3g, sodium bicarbonate 20 g, made of effervescent tablets conform to the requirements of the 2010 edition of pharmacopoeia.