Study On Preparation And Depth Development Of Pepper Seed Oil

China is the origin of the Chinese prickly ash. There is the largest planting area and production of the Chinese prickly ash. In China, the Chinese prickly ash cultivation area had reached more than 20 million acres only in 2009, and the annual production capacity was 1 million tons. The Prickly ash seeds as the main by-products, theoretically harvests beyond about 20% to the peel. If abandoned the Prickly ash seed, the environmental influence cannot estimate.On the basis of earlier stage Extraction of Crust Oil and Kernel Oil from Pepper seeds by Two-step Extraction Technology and Crust Oil translates to Biodiesel by Enzyme Catalysis, this paper studied the prickly ash seeds oil comprehensive utilization and kernel oil depth development process. In these aspects of the acid and alkaline process to remove prickly ash seeds crust oil, the traditional method of spiral squeezing the prickly ash seeds kernel oil, the Prickly ash seeds kernel oil α-linolenic acid bag and separation, and the Prickly ash seeds oil composition high performance liquid determination methods, this paper had system research.(l)Study on the acid process to remove the prickly ash seed crust oilThe prickly ash seeds contained kernel (the whole seeds) as raw material reacted with anhydrous ethanol under the catalysis of concentrated sulfuric acid, in order to translate the prickly ash seed crust oil to fatty acid ethyl ester which could be used as biodiesel. The result showed that, when the catalyst dosage was 1% (catalyst/ anhydrous ethanol volume ratio),the reaction temperature was 80℃, the ethanol dosage was 400 ml, the reaction time was 60 min, the prickly ash seeds crust oleic acid ethyl ester conversion rate reached 88.56%. At the same time, the oleic acid ethyl ester high-performance liquid chromatography (HPLC) was established. The determination conditions were as follows:C18 reversed-phase chromatography column (150 mm x 4.6 mm,5 μ m), the mobile phase was methanol-88%acetonitrile solution= 50:50, the ultraviolet detected wavelength was 210 nm, the velocity was 0.6 ml/min, the column temperature was 25℃, and the sample injection dosage was 20 μl.(2)Study on the alkaline process to remove the prickly ash seed crust oilThe prickly ash seeds contained kernel (the whole seeds) as raw material reacted with Sodium hydroxide-aqueous solution to translate the prickly ash seed crust oil to fatty acid sodium salt which could be used as industrial detergent. The result showed that, when the reaction temperature was 60 ℃, the concentration of Sodium hydroxide-aqueous solution was 1.4% (sodium hydroxide/water quality ratio), the reaction time was 30 min, the alkali liquor repeated use was 5 times, the removal rate of the whole prickly ash seeds crust oil reached 93.85%. At the same time, the enlargement test was carried out which could dispose 50 kg prickly ash seeds one-time. The crust oil removal rate was 91.16%.(3)Study on the Prickly ash seeds kernel oil squeezingAfter the alkaline process enlargement test, the prickly ash seeds which were removed crust oil squeezed the kernel oil by the traditional spiral squeezing method. After squeezing repeatedly the kernel oil yield reached 20.2%.There was no difference between squeezing kernel oil and soaks raises kernel oil in the fatty acid content, while the squeezing kernel oil with full-bodied fragrance was no solvent residual, and the colloid content were low. The squeezing kernel oil was a kind of natural green health food.(4)Study on the depth development of Prickly ash seed oilThe prickly ash seed oil was used as raw material, to bag and separate a-linolenic acid by ultrasonic-assisted hydroxypropyl-β-cyclodextrin embeddedness method. The result showed that, when the ultrasonic power was 300 W, the ultrasonic time was 20 min and the ethanol+FFA:HP-β-CD mass ratio was 1:8, the a-linolenic acid inclusion rate reached 90.26%,the sample of α-linolenic acid content was 92.58%.Using this process conditions to squeezing kernel oil, the sample of α-linolenic acid content was 92.93%.At the same time, the α-linolenic acid and linoleic acid high-performance liquid chromatography (HPLC) was established. The determination conditions were as follows:C18 reversed-phase chromatography column (150 mm × 4.6 mm,5 μ m), the mobile phase was methanol-0.1% phosphoric acid solution= 90:10, the ultraviolet detected wavelength was 205 nm, the velocity was 1.0 ml/min, the column temperature was 25℃, and the sample injection dosage was 10 μl.