The Research Of Preparation And Antibacterial Ability Of Silver Incorporated Titania Implant Antibacterial Coating Materials

Objective:Using a homogeneous precipitation method, micron-sized antibacterial titanium dioxide spheres with high specific surface were prepared by doping nano-titanium dioxide with silver particles having stable performance and broad-spectrum antimicrobial ability. Titanium dioxide microspheres not only possess the performance advantage of nano-materials, and overcome the biosafety issues by nanometer size effect. Antibacterial effect against Staphylococcus aureus by antimicrobial microspheres particles of silver doped Titanium dioxide was studied in dull conditions. In order to provide the antimicrobial implant material a new choice and theoretical basis.Method:This study could be divided into three parts:1. Synthesis of silver incorporated titania antibacterial microspheres particlesTitanium sulfate was used as titanium source, silver as silver nitrate source, urea as a precipitating agent, polyvinylpyrrolidone(PVP) and sodium dodecyl sulfate(SDS) as a stabilizer, respectively. With the homogeneous precipitation method,90 o C under heating conditions, silver incorporated titania antibacterial microspheres particles were made. Products were divided into PVP group and SDS group. Change the amount of silver sources to get antibacterial microspheres particles with different contents of Ag.2. Physical and chemical characterization of silver incorporated titania antibacterial microspheres particlesField emission scanning electron microscope(FESEM) and high-resolution transmission electron microscopy(HRTEM) were used to observe the crystal morphology and microstructure of products. X-ray powder diffraction(XRD) was used to study crystal phase. Nitrogen(N2) adsorption/desorption on the samples wasmeasured by a micrometrics surface area and porosity analyzer. X-ray spectroscopy(EDAX) and X-ray photoelectron spectroscopy(XPS) were utilized to obtained qualitative and quantitative analysis of the chemcial elemental composition of the products.3. Antibacterial performance of silver incorporated titania antibacterial microspheres particles in darkNo light conditions, silver incorporated titania antibacterial microspheres particles in PVP group and SDS group with different amounts of silver doped were adsorbed on round blank filter papers as an antimicrobial agent and to S. aureus bacteria as the goal bacterial. Through the inhibition zone test, the diameter of the inhibitory rings were measured and the antibacterial activity was evaluated and compard.Result:1. Titania antimicrobial particles incorporated with silver in PVP and SDS groups are made by Ag, Ti O2 nanocrystals deposited by microspheres, microspheres having a diameter of 1 ~ 4 mm. Where in the Ag nanocrystals in elemental form,Ti O2 anatase nanocrystals, Ag nanocrystals evenly distributed between Ti O2 nanocrystals.2. PVP group and the SDS Group microspheres containing silver antibacterial titania particles have high specific surface area.3. In dark, samples both in SDS and PVP group do show antibacterial effect.With the increase of Ag doping, zone diameters of samples in SDS and PVP group are gradually increased, and the comparison results of any two groups with different silver doping were statistically significant(p <0.05) with exclude of groups with silver doping amount of 0.5~0.8. When Ag doping amount is the same, the inhibition zone of SDS group was significantly bigger than the diameter of PVP group, the difference was statistically significant(p <0.05).Conclusion:With PVP or SDS as stabilizer, obtained by homogeneous precipitation method,Ag / Ti O2 antimicrobial microsphere particles were composed of Ag and Ti O2 nanocrystals. The method is simple and low cost of raw materials. The antibacterial microsphere particles possess both the advantage of properties of nanomaterials and to some extent, overcome the nanometer size effect which may bring biosafety issues.Microspheres of antimicrobial particles having a high surface area, increase the contact chance for bacteria and antimicrobial microsphere particles which facilitate the antibacterial performance. Antibacterial experiments proved that in the dark condition, Ag / Ti O2 antimicrobial microsphere particles synthesized by the method with PVP or SDS as stabilizer showed significant antimicrobial properties. The microstructure characteristic of the Ag / Ti O2 antibacterial microsphere particles was the main reason for antimicrobial properties in dark of the materials.