Preparation And Application In Food Safety Of Molecularly Imprinted Polymers With Rosin-Based As Solid-Phase Sorbent For Basic Orange Extration

In this study, molecularly imprinted polymers(MIP) of Basic orange were first synthesized by aqueous micro-suspension polymerization, using Basic orange Ⅱ as the template molecule, acrylamide (AM) and modified rosin as the functional monomer and the cross-liner, respectively. The optimum synthetic conditions were selected and evaluated by binding analysis to screen out the appropriate MIP for the following tests on the physical characteristics, adsorptive ability and selectivity. Using the basic orange-imprinted polymer as a solid phase extraction(SPE) sorbent, a novel sample pretreatment technique that can be coupled to high performance liquid chromatography(HPLC)had been developed for the determination of basic orange Ⅱ in food. The results were as follows:1. The optimum synthetic conditions were confirmed by single factor experiment, and its repeatability was proved good by repetitive experimental. The polymers synthesized using cross-linker modified rosin had the highest crosslinking degree and physical characteristics steady than TRIM, EDMA and MBA.2. The structure and physicochemical performance of the polymer were investigated by UV, FT-IR, XRD, elementary analysis, BET, SEM, et al. The results indicated that surface areas, the average pore diameter were 0.1786 m2/g, 8.21 nm and the SEM show that the particle of the MIP whose surface is full of dense caities was round.3. The major factors that influence the characteristic sorption for basic orange Ⅱ were discussed. The Scatchard analysis result suggests that there were two classes of binding sites between basic orange Ⅱ and AM, and the value of Bmax is 118.17 mg/g. (Bmax is the apparent maximum amount that the MIP can be bound).4. Molecular recognition of MIP was discussed by the experiment of characteristic sorption and selecting sorption, and the data show that for the NMIP, the absorption of the substrate is mainly non-specific, while the MIP has a specific adsorption to basic orange Ⅱ.5. Using the basic orange-imprinted polymer as a solid phase extraction(SPE) sorbent, a novel sample pretreatment technique that can be coupled to high performance liquid chromatography(HPLC)had been developed for the determination of basic orange Ⅱ in food.Basic orange imprinted polymer was used as the sorbent of solid-phase extraction(MISPE). The conditions of MISPE were selected by HPLC. The results of Standard addition method recovery experiment showed that the extraction recoveries of the basic orange Ⅱ between 68.43% and 80.25%, and the RSD% is 0.15%～1.17%, indicating that the MISPE-HPLC can be effective to extract basic orange Ⅱ in food. HPLC coupled with MISPE was used to determinate basic orange Ⅱ in Eight kinds of food bought in market, Not found positive samples.6. For the basic orange extraction, off-line SPE was developed with the MIP as sorbent. The performance of MIP was compared with that of NMIP and Cig simultaneously, In this work, MISPE with class-specificity and class-selectivity for the target analyte of basic orange Ⅱ were developed. The binding performance of NISPE and C18 column for basic orange Ⅱ and rhodamine B was non-specific adsorption.