Study Of Rosin-based Macromolecularly Imprinted Polymer As The Stationary Phase In High Performance Liquid Chromatography For Selective Separation Of Alkaloid

At present, alkaloid were extracted, separated and purified efficiently from the plant by molecular imprinting technique, which has been one of hotspot of molecular imprinting technique application.A kinds of rosin-based molecularly imprinted polymers were prepared by suspension polymerization in the aqueous phase using berberine hydrochloride, tetrahydropalmatine and hydroquinine as the template, respectively, methyl acrylic acid as a functional monomer, and ethylene glycol maleic rosinate acrylate, which contains a phenanthrene ring skeleton, and ethylene glycol dimethacrylate as combinatorial cross-linkers. The different template molecular imprinted polymers were successfully used as a selective stationary phase in high performance liquid chromatography.The preparation, characterization and application of berberine hydrochloride molecularly imprinted chromatographic column were studied. The separation performance of a chromatographic column was determined from the selectivity (evaluated by separation factor) and sorption selectivity (evaluated by imprinting factor) of the molecularly imprinted and non-imprinted polymers toward the template. The results showed the maximize separation and imprinting factors were investigated on optimum conditions. The highest imprinting and separation factors obtained were 1.924 and 18.52, respectively. Simultaneously, the chromatographic column backpressure was stable and good permeability. The chromatographic column was used to separate template molecules from coptis root extract, which the column did effectively. The results illustrate the chromatographic columns have high selectivity and excellent affinity.The preparation, characterization and application of tetrahydropalmatine molecularly imprinted chromatographic column were studied. The separation performance of a chromatographic column was determined from the selectivity (evaluated by separation factor) and sorption selectivity (evaluated by imprinting factor) of the molecularly imprinted and non-imprinted polymers toward the template. The results showed the maximize separation and imprinting factors were investigated on optimum conditions. The highest imprinting and separation factors obtained were 2.784 and 3.835, respectively. The chromatographic column can separate mixtures of template molecules and analogue effectively. Such chromatographic columns with high selectivity and excellent affinity can be used to separate and detect tetrahydropalmatine from other compounds.The preparation, characterization and application of hydroquinine molecularly imprinted chromatographic column were studied. The separation performance of a chromatographic column was determined from the selectivity (evaluated by separation factor) and sorption selectivity (evaluated by imprinting factor) of the molecularly imprinted and non-imprinted polymers toward the template. The results showed the maximize separation and imprinting factors were investigated on optimum conditions. The highest imprinting and separation factors obtained were 1.749 and 9.579, respectively. The chromatographic column can separate mixtures of template molecules and analogue effectively. Such chromatographic columns with high selectivity and excellent affinity can be used to separate and detect hydroquinine from other compounds.