Community Structure And Distribution Characteristics Of Sulfate-Reducing Bacteria In Wuliangsuhai Wetland

Wuliangsuhai, a typical asakusa lake on Inner Mongolia plateau, faces the eutrophication, soil salinization and other environmental issues. Meanwhile, the growth of industrial and agricultural practices, together with the continuous growth of population and urban development, has caused a significant destruction of Wuliangsuhai wetland. As an important participator in carbon and other elements cycles, sulfate-reducing bacteria (SRB) plays a significantly important role in reducing the sulfate decomposition and H2S releasing. The study of community structure and abundance of SRB in wetland environment can provide new insights and add scientific valuable reference for exploration microbial community structure and distribution characteristics. Meanwhile it provides the basis for the restoration of wetlands.Using clone library construction and real-time quantitative PCR techniques, we setted up a landward transect in Wuliangsuhai Xiaohekou plots to study the diversity and abundance of SRB. The main results as follows:The diversity of SRB in sediment environment is higher than other plots. Diversity of SRB are the lowest in nitraria desert soil. SRB communities are subjected to the Proteobacteria phylum, including five familia: Acidithiobacillales, Desulfurella, Chromatiales, Desulfovibrionaceae and Desulfobacteriaceae. And the dominant microfloras are Chromatiales, Desulfovibrionaceae and Desulfobacteriaceae.According to the results of Quantitative PCR, the abundance of apsA in four studied plots has great difference, and ranges from 2.37X 104～1.34X 107 copies/g.According to the results of PC A, the main impact factors of the SRB distribution are the content of relative moisture and ammonium nitrogen, which have the significant influence on the community structure of SRB in sediment environment. The total water soluble salt has significant effect on the community structure of SRB in suaeda salinization meadow soil and nitraria desert soil environment. The content of sulfate has little influence on the diversity of SRB.