Compared Of Toxicity On Takifugu Fasciatus Exposed Of Phenanthrene,3-methyl Phenanthrenen And Phenanthrenequinone

The polycyclic aromatic hydrocarbons (PAHs) are one of the ubiquitous contaminants in environments. Crude oil is composed of a complex mixture of hydrocarbons, especially the alkyl-polycyclic aromatic hydrocarbons, which make up more than 80% of the toxic components of crude oil. Oxygenated-PAHs are important transformation product of PAH in environments. However, at presents, there is few study on the toxicities and mechanism of action of alkyl-PAHs and oxy-PAHs. The aim of this thesis was to study and compare the activities of enzymes, oxidative stresses and metabolism responses of marine economic fish species, Takifugu fasciatus,which exposed to three compounds:phenanthrene,3-methyl phenanthrene and phenanthraquinone The main obtained results were as follows:1. The enzymes activities were evaluated after 7d exposure of T. fasciatus to phenanthrene,3-methyl phenanthrene and phenanthraquinone. Results showed that the ethoxyresorufin O-deethylase(EROD) activity was induced by high concentration phenanthrene and inhibited by phenanthraquinone reversely.There was no significant induction of EROD activity exposed to 3-methyl phenanthrene. All three compounds could significantly induce GST activities at higher concentration. However, compared with GST activity, the inductive effects of LDH activity by three compounds were not quite sensitive to except for 200μg/L of phenanthrene treatment.2. The MDA levels in hepatic cells indicated that all compounds could result in an obvious oxidative damage, while under the same concentration, the effect of 3-methyl phenanthrene was stronger than that of phenanthrene.3. The residual concentration results of parent compounds and metabolites in bile and liver demonstrated that metabolite content in liver had significant correlation with concentration, while metabolites in bile increased with the increase of concentrations of three compounds. The fluorescence spectra indicated that the metabolites instead of their parent compounds, were the mostly dominant components existing in the bile.4. The ultrastructure observation of T.fasciatus hepatic cells afer exposure to three compounds showed a serious damage of hepatocyte structure.The hepatic lesions were characterized by the damage of mitochondrion and endoplasmic reticulum exposed to higher concentration of phenanthrenen and 3-methyl phenanthrene. In comparison, phenanthraquinone induced the edema of mitochondria and endoplasmic reticulum with increasing concentration, indicating a severe impact on organelle.