Effect Of Silver Nanoparticles And FA On The Cytotoxicity And RNS/ROS Production Of A_L Cells

With the development of nanotechnology, nanomaterials have potential toxicity to ecological environment. Silver nanoparticles (nAg) are widely used in the fields of medicine, biology and daily life because of their excellent antimicrobial, electrical and optical properties. Therefore, the products will release nAg into the environment, nAg’s ecological security should be noticed by us. Fulvic acid (FA) exist ubiquitously in aquatic environments and have a variety of functional groups. However, little is know about their potential impacts on nAg.it may nAg interaction. The nAg biological toxicity would be changed by this interaction.To explore the influence of silver nanoparticles (nAg) and nAg associated with fulvic acid on the relationship between proliferation and RNS/ROS of Human-hamster hybrid AL cells in vitro. AL cells were exposed to nAg (0.1-5 μg/ml) and silver nanoparticles associated with fulvic acid (10 μg/ml FA) for 24 h. Cell viability was examined by ALamar Blue assay. Reactive nitrogen species (RNS) was examined by fluorescent probe with Inverted fluorescence microscope. Reactive oxygen species (ROS) was examined by DCFH-DA fluorescent probe with a microplate reader. Because nAg could be dissolved into silver ions (Ag+) from nAg in culture medium, the study explore the influence of Ag+ on the proliferation and RNS/ROS of cells in vitro. Silver ions and nAg can inhibit the proliferation cells. Silver ion released from nAg solution had much more effect on cell vitality than nAg at low concentrations. The nAg had much more effect on cell vitality at high concentrations. RNS production was significantly induced which is mainly caused by silver ions. RNS levels were a little increased in cells upon silver nanoparticles mixture with FA treatment, compared to the untreated cells. ROS production was significantly induced which is mainly caused by nAg. ROS levels were slightly increased in cells upon silver nanoparticles associated with FA treatment, compared to the untreated cells. AL cells suffered from enhanced oxidative stress, as evidenced by increased levels of ROS, RNS and consequentially reduced proliferation. Because FA inhibited the production of free radicals to a certain extent, cell vitality was not significantly reduced.