Study On Artemisia Seeds Containing Material And The Purification Of Its Polysaccharide With The Structure And The Antioxidant Activities Of ASP-1

Artemisia desertorum Spreng. Syst. Veg(A.s.v) belongs to Artemisia Compositae perennial, semi shrubby plants, mainly grown in the desert regions of our country. It has environmental protection function in desertification areas and can promote the development of economy. It is an important Chinese herbal medicine resources as well. A.s.v seed polysaccharide is extracted from A.s.v. It has unique physiological structure and activity, which has realistic significance to research. At present, the domestic and foreign research on A.s.v seed polysaccharide is less. This paper analyzed the A.s.v seeds polysaccharide, trace minerals elements and protein content. In this paper the extraction technology of A.s.v polysaccharide was studied and optimized. The structure, molecular weight, connection of purified A.s.v seed polysaccharide ASP-1 was investigated. It also explored the antioxidant activity of A.s.v polysaccharide ASP-1. The main results are as follows:The trace mineral elements and protein in A.s.v seeds was determined by ICP-MS and Automatic Kjeldahl apparatus. The results showed that A.s.v seed contains rich essential elements, for example. Fe, Cu, Zn, Cr, Ni, V, Se, Co, Mn,Sr.The protein content of Artemisia seeds was 25.4%;This chapter determined the A.s.v seed polysaccharide, compared water and ethanol extraction method in order to obtain the optimum extraction technology of A.s.v seed polysaccharide and the extraction process of A.s.v seed polysaccharide. The results showed that the optimum extraction condition of pretreatment of A.s.v polysaccharide:solid-liquid ratio was 0.20 g/ml, the water bath temperature was 79 ℃, the water bath time was 45 min, under this condition, the Artemisia seed polysaccharide yield was 5.82%.DEAE-52 cellulose exchange column was used to separate and purify A.s.v seed polysaccharide. It showed that the polysaccharide after separation with four components:ASP-1, ASP-2, ASP-3 and ASP-4. The highest content components was ASP-1, which was isolated and purified from Sephadex-100 column again. This study used the color experiment to determine the physicochemical properties of A.s.v seed polysaccharide. The results showed that the A.s.v seed polysaccharide separated into their pure components after impurity removal. The polysaccharide ASP-1 had homogeneity, ASP-1 was purified through UV and was a reducing sugar without ketone group and it was a kind of neutral sugar. It analyzed the structure and functional groups of ASP-1 by infrared spectroscopy. Absolute molecular weight and relative molecular weight in ASP-1 was investigated by using high molecular gel permeation chromatography and Ubbelohde viscometer investigate. To determinate polysaccharide molecular bond position and functional group structure, The pre-column derivatization and GC was used to analyze the composition of monosaccharide in ASP-1. The Mw of ASP-1 was 2.406×104g/mol. The Mn is 2.029 X 104g/mol and the Mv was 2.05 X 104g/mol. The molecular structures existed of 1â†'6 glycosidic bond or non reducing end, the main connection of the monosaccharide was 1â†' or 1â†'6 glycosides bond, and there may be had some 1â†'2,1â†'2,6 or 1â†'4,6 glycosides bond and so on. ASP-1 mainly had L-arabinose, L-rhamnose, D-mannose, D-galactose, D-glucose, and the molar ratio is 0.38:0.38:0.5:0.77:0.81:1;This paper analyzed and compared the antioxidant ability of DPPH free radical, hydroxyl radical, superoxide anion free radical, and antioxidant function in (3-carotene integrin oleic acid system analysis.The results showed that the purified and non-purified A.s.v seed polysaccharide had antioxidant capacity. ASP-1 scavenging ability of DPPH free radicals, hydroxyl radicals, superoxide anion can reached more than 50%. Antioxidant capacity of A.s.v seed polysaccharide ASP-1 in (3-carotene integrin oleic acid system reached 40.2%, indicating the A.s.v seed polysaccharide with antioxidant activity.