| Cordyceps militaris is a kind of food and medicine fungi which belong to the same species with Cordyceps sinensis. Research shows that Cordyceps militaris and Cordyceps are extremely similar with the active component and pharmacodynamic action. The main active components of Cordyceps militaris is mainly Cordyceps polysaccharide, adenosine kind, mannitol, SOD enzme and some trace elements. The reports about Cordyceps militaris are mainly concentrated in the cordycepin and Cordyceps polysaccharide, rarely related to the protein and relative health care products.Taking Cordyceps militaris as the raw material, then determining the basic composition and the main functional component, result shows that some of the main components such as protein content is 28.73%, Cordyceps polysaccharide is21.85%, adenosine content is 1.20%, Cordycepin is 1.08% and mannitol content is 3.824%.The Cordyceps militaris protein was extracted and precipitated through alkali extraction and acid precipitation. Single factor and orthogonal experiment were used to optimize the extracting conditions. The optimum conditionswere that the concentration of NaOH solution was 0.15mol/l, the ratio of solid to liquid was 1:17.5 and the time was 120min. Using acid precipitation to subsidence protein, result showed that pH4.6 is the settlement point ofprotein.Selecting compound protease, using the degree of hydrolysis as the hydrolysis index and optimizing the hydrolysis conditions by the means of single factor and orthogonal experiment, ultimately determined the optimum conditions:the hydrolysis time is 7h, pH is 9.15, hydrolysis temperature is 52.59℃ and the degree of hydrolysis is the maximum in this case. The enzymolysis liquid was freeze-dried.We used the Sephadex Gel chromatography and Tricine-SDS-PAGE electrophoresis, aiming at separating the Cordyceps militaris peptides and analyzing molecular weight distribution range of Cordyceps militaris peptides. The results of Tricine SDS-PAGE electrophoresis showed that the molecular weight distribution range of Cordyceps militaris peptides was mainly distributed in 4722-7547Da. The consequence of Gel chromatography displayed that the molecular weight of Cordyceps militaris peptides of 296-5343 Da.At the vitro chemical simulation system, we admeasured the superoxide anion free radical (O2-) clearance ability, DPPH-scavenging ability and the hydroxyl radical (-OH) inbibiting ability of Cordyceps militaris peptides. Experimental results showed that when the Cordyceps militaris peptides concentration was 1.Omg/ml, the superoxide anion radical clearance rate was 37.80%, the DPPH-scavenging rate was 46.34% and the hydroxyl radical inbibiting rate was 43.21%. It proved the cordyceps militaris peptides by the mean of compound protease enzyme hydrolysis had the ability of scavenging free radicals and anti oxidation.The plate filter method was adopted to examine bacteriostasis ability of the Cordyceps militaris peptides. Ultimately the results showed that the bacteriostasis results of the Cordyceps militaris peptides on Staphylococcus aureus Rosenbach, Escherichia coli, Bacillus subtilis Cohn and Salmonella Lignieres were negative. These indicated that of the Cordyceps militaris peptides had no antibacterial activity to the above four kinds of bacteria.The processing technology of functional oral liquid was studied. The polypeptide, which was made by protease enzymolysis, adding the activated carbon, beta cyclodextrin and malate todebitter treatment, adding the sugar, critic acid, honey and glycine, was determined to the optimal formula of composite be sensory evaluation indicators. The addition amount of the four substances were sugar 10%, honey 3%, critic acid 0.2% and glycine 0.1%. |
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