Characterization Of Heparin-folate Nanoparticles And The Research On Targeting

Folate receptor (folate receptor, FR) is a single-chain transmembrane glycoprotein, which can be overexpressed in most epithelial malignancies cells, but low expression in normal cells. Folic acid - drug conjugates of folic acid and tumor cell surface receptor-specific binding after by endocytosis (endocytosis) into tumor cells, enabling targeted therapy of tumors. Thus, folate become a widely used anti-tumor nanomedicine.Based on heparin-folic acid, a novel nanoparticles was prepared and characterized and we found that only 15.1-18.1% folate acid was on the surface of nanoparticles. The animal and cell imaging experiments showed that the target effect was not disappeared. Because folic acid is widely used in anti-cancer nanomedicine, the results have great importance and significance.Mainly for this paper are listed as follows:(1) Natural polymer by chemical modification of folate connection method, a novel, spherical heparin - folate nanoparticles was prepared.(2) The self-assembly mechanism of heparin-folic acid nanoparticles was further discussed, and the percentage of surface folic acid was measured through UV - NMR method, we found that the surface folic acid self-assembled was about 15.1%-18.1%.(3) Self-assembled heparin-folic acid nanoparticles, folate targeting doesn’t disappear, also Nanoparticles can physically package fluorescent dye. We designed a kind of fluorescent probes for in vivo imaging.