| Deer antler base, commonly known as Lu Hua Pan, Lu Jiao Tuo Pan in Chinese, is the ossified and rudimental antler of the male sika deer and red deer after sawing off the velvet antler, which then falls off by itself in the following year in the spring when the new velvet antler begins to germinate. It is stronger than the velvet antler. Deer antler base has been recorded in the Chinese medical classics Shen Nong Ben Cao Jing and is believed to nourish the Yin, tonify the kidney, invigorate the spleen, strengthen bones and muscles, and promote blood flow. Deer antler base was as the byproducts of the traditional Chinese medicine herbs-the velvet antler,its drug effective value has been ignored always so that the bioactivity and ecnomic value of deer antler base has not been used fully and lead to polluting the environment and wasting the source of the protein.Terefore, deer antler base powder as materials were studied for the polypeptide in the deer antler base.Firstly, using the method of double enzyme hydrolysis to prepara deer antler base polypeptide and optimizing the extracting process,then,studying the vitro antioxidant activity,finally, after the freeze-drying process,exploring the extracting polypeptide of deer antler base if have the treatment to gland mammary through animal experiments.The results are as follows:1 In this study, antlers base powder as raw material, the degree of hydrolysis was as a measure of the optimal indicators of the extraction process, And the best combination of dual enzymatic hydrolysis of peptides was selected and obtained.Based on one-factor-at-a-time method, the response surface analysis method based on Box-Behnken design was applied. The result of determining the optimal level of enzyme hydrolysis process is:temperature of 49℃, pH of 8.0,270 min with enzyme dosage of 11000 U/g, enzyme activity ratio of (papain: trypsin) 2:1 and a substrate concentration of 6 g/100 mL. Under this condition, the hydrolysis degree could be reached 15.91%, peptide yield could be reached 65.10% respectively.2 By anti-oxidation experiments to study deer horn disc polypeptide hydroxyl radical (OH) and the ability to inhibit DPPH-radical scavenging activity, and ascorbic acid Vc comparing experimental results show that:in the peptide concentration reached a maximum 1.0μg/mL, the deer horn disc polypeptide hydroxyl radical (·OH) inhibition rate reached 91.51 percent, close to the rate of inhibition of ascorbic acid, while DPPH-scavenging rate was 19.6%.3 Deer horn disc polypeptide pH stability study results show that:when the temperature is 37℃, deer horn disc polypeptide more stable in the range of pH6.0-8.0, when pH<6.0 and pH>8.0, the stability of the disk obviously antlers reduce, at pH 2.0, the variability rate of up to 45.76%, the oxidation resistance was reduced to 32.88%; when the pH value of 12.0, the maximum rate variability reached 58.32%,30.08% reduced oxidation resistance.4 Deer horn disc study results show that the thermal stability of the polypeptide:in the range of pH6.0-8.0 range, when the reaction temperature of lower than 60℃, better stability. When the pH<6.0, the thermal stability of the polypeptide antlers plate with the decrease of pH decreases when the pH 2.0,70℃ treatment 20min, the highest rate of 59.97% denaturation, oxidation resistance is reduced by 41.91%; when the pH>8.0, the thermal stability of the polypeptide antlers plate with increasing pH value decreased, pH 12.0, after 70℃ treatment 15min, degeneration reached the maximum 55.04%,38.56% reduced oxidation resistance.5 Deer horn disc biologically active polypeptide in vivo results show that:the experimental system through animal experiments, the use of exogenous hormones induced by mouse mammary hyperplasia model, and each experimental treatment group of mice fed different doses of the polypeptide deer horn disc by weight change observed in mice, serum estradiol (E2), progesterone (P), prolactin (PRL), luteinizing hormone (LH), total superoxide dismutase (SOD), malondialdehyde (MDA) and change the content as well as changes in organ index of glutathione peroxidase (GSH-PX) through the analysis of breast tissue biopsy results showed that:When deer horn disc polypeptides dose of 125mg/kg, deer horn disc polypeptide can significantly improve the mouse thymus, pancreas coefficient, reduced ovarian coefficient, when the dose of 200mg/kg, deer horn disc polypeptide is capable of reducing the uterus coefficient mice improved SOD hyperplasia mice, GSH-PX activity, decrease MDA content; reduce serum E2, PRL content, increased serum P, LH content; can significantly inhibit the proliferation of the symptoms of breast tissue, making glands and glands bubble and so on back to normal, reduce the number of acinar atrophy, ease gland catheter lumen expanding phenomenon. This shows that deer horn disc polypeptide is regulated by the body’s overall level of immunity, strengthen the response to exogenous and self-healing injury, reduce the extent of tissue damage, by regulating the hypothalamus-the gland secretion system, restore estrogen levels, so that breast tissue involution the ability to return to normal, control and reduce the proliferation of abnormal gonadal organ and tissue type, and use a good anti-inflammatory properties, eliminating inflammation, which played a role in the treatment of breast hyperplasia. |
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