The Controllable Preparation Of Graphene Oxide And The Properties As Drug Carrier

Malignant tumor, a disease that could badly threaten people’s health, became the first murderer in the world. With the development of technology, various tumor treatments were emerging such as operative treatment, chemotherapy, tumor radiotherapy and so on. Among these methods, chemotherapy was the primary approach. As for chemotherapy, there were several impediments for their use in cancer therapy such as poor solubility in water and side-effects. Furthermore, low specificity turns out to be a key problem needed to overcome. With the development of nanoscience and nanotechnology, a series of new nanomaterials have been synthesized that leads to the development and research of ness DDSs. Nanomaterial-based drug carriers with efficient loading, target delivery and controlled release of drugs have become a hot spot in the field of biomedicine research.In recent years, graphene for drug loading and delivery has attracted great interest. In this work, we focus on the synthesis of graphene oxide-based drug carrier and characterization of its microstructure, morphology and cytotoxicity. A new drug carrier based on removal of carboxyl groups from graphene oxide (GO-RCOOH) with small size has been obtained.In summary, we carried out our experiments from the following aspects:1) Synthesis and characterization of graphene oxide having different degrees of oxidation. Modified Hummers’method was used, by changing the amount of the precursor, potassium permanganate oxidation, to regulate graphene oxide. The results showed that the prepared graphene oxide was composed of carboxyl, hydroxyl and carbonyl groups and other oxygen-containing functional groups, with C/O ratio ranging from 1.975 to 3.305.2) For the above graphene oxide material, the ultrasonic time has been studied to regulate the GO size. The optimized degree of oxidation and GO size were determined based on the study of drug loading efficiency and cytotoxicity.3) For the above optimization graphene oxide material, removal of carboxyl groups treatment (GO-RCOOH) was first carried out, then the cytotoxicity for U251, finally doxorubicin hydrochloride (DOX) drug loading experiments. GO materials prepared using a normal process has been chosen as controlled sample for comparative analysis of cytotoxicity and drug loading efficiency.4) Composited the above optimization graphene oxide with chitosan to primarily study load efficiency of DOX.