Application Of Chemical Oscillating Reaction In The Analysis Of Medicine And Natural Antioxidant Substances

The chemical oscillation reaction is widely existed in industry.This reaction is of important theoretical significance, and wide application prospect in the actual life. With high sensitivity and simple equipment, chemical oscillation method has attracted more and more attention in recent years. In this paper, the disturbances of different drugs and natural antioxidant substances on chemical oscillation systems were studied, the mechanisms of the reactions and disturbances in the oscillation system were also discussed. The main work is as follows:1. A new method for determination of troxerutin was developed based on the disturbance of troxerutin to Ma-H2SO4-Ce4+-KBrO3 oscillation system. Under optimal conditions, the variation of ΔE had a linear relationship with troxerutin concentration in the range of 0.808～40.4 μmol·L-1, the detection limit was 2.14×10-7 mol·L-1. The method was used to determine troxerutin in injection, the recovery was 98.8～101%. It was showed that, the results of this method had no significant difference with UV method. The possible mechanism was studied by cyclic voltammetry and UV spectrophotometry.2. A new method for determing the antioxidant activity of common traditional Chinese medicine and herbal tea was developed based on the disturbance of the antioxidants to the system of Ma-H2SO4-Mn2+-KIO3-H2O2. Under the optimized conditions, the concentration of the antioxidant compounds had a linear relationship with the inhibition time. The effect of the compatibility of traditional Chinese medicine on the antioxidant activity was also investigated. The possible mechanism was studied using flow injection chemiluminescence method.3. Based on the disturbance of VB12 on Ma-H2SO4-Mn2+-KIO3-H2O2 system, a new method for determination of VBi2 was developed. Under optimal conditions, the variation of ΔE had a linear relationship with VB12 concentration in the range of 0.123～21.1 μmol·L-1, the detection limit was 1.02 nmol·L-1. The recovery of VB12 in injection was 97.8%～103%, and the results obtained by this method had no significant difference with those obtained by UV method. The possible mechanism was discussed.4. A new method for determination of etamsylate was established based on the disturbance of etamsylate to H2SO4-Mn2+-Malic acid-KBrO3 system. Under optimal conditions, the variation of ΔE had a linear relationship with etamsylate concentration in the range of 9.43×10-6～2.01×10-4 mol·L-1, the detection limit was 2.23×10-6 mol·L-1. The recovery of etamsylate in injection, was 97.0%-103%, and the results of this method had no significant difference with those obtained by UV method. The possible mechanism was studied using cyclic voltammetry and UV spectrophotometry.5. A new method to determine noradrenaline bitartrate was established based on the disturbance of noradrenaline bitartrate to H2SO4-Mn2+-Ma-KBrO3 system. Under optimal conditions, the variation of AE had a linear relationship with noradrenaline bitartrate concentration in the range of 0.409-40.9 μmol·L-1, the detection limit was 1.18×10-7 mol·L-1. The recovery ratio was 94.9%～01%. It was showed that there was no significant difference between the results obtained by the method and those obtained by UV method. The possible mechanism was studied using cyclic voltammetry and UV spectrophotometry.