Study On The Separation Behavior Of Medicago Sativa、Mulberry Of Leaf Protein With Foam Method

Leaf protein is the protein that is extracted from stem and leaf of plant. It is a rich source and rich source, but no cholesterol. People can enhance the antisenility and their health effects by eating it. leaf protein not only are all good sources of amino acid to meet the needs of the human body, but also vitamin and trace elements. Thus it have high exploitable. Leaf protein development and utilization of leaf protein can alleviate the people’s demand for protein, and at the same time, improve the utilization value of leaf protein and has a certain economic effect; Foam separation method is a new separation technology, and compared with the traditional extraction method, combining the factors that can be extracted to improve separation effect. This experiment is using mulberry leaf and alfalfa as raw material, to recover the enrichment ratio as an indicator of leaf protein foam separation. The experimental results are as follows.First, medicago sativa leaf and mulberry leaf as material Technology optimization of Medicago sativa leaf protein separation:Medicago sativa leaf was shattered by grinder and sized by 60 mush. 12.5g of Medicago sativa powder, With 0.7% sodium. Hydroxide solution as the extraction agent, under the condition of temperature 60 ℃and the ratio of material to solvent of 1:20(g/v), in a stirring for 20 minutes, was extracted twice and combined filtrate to be used. Second The foam fractionation of Medicago sativa leaf protein was optimized by response surface methodology. Based on the response surface and contour plots established with protein recovery and enrichment ratio as the response values, the optimum conditions for the separation of Medicago sativa leaf protein were found to be pH value of 7.0, protien concentration of 87.5 mg/L, the loading liquid volume of 600 mL, the air flow rate of 200 mL/min. The recovery of Medicago sativa leaf protein was 90.2% and the enrichment ratio was 7.64; Technology optimization of Mulberry leaf protein separation First Mulberry leaf was shattered by grinder and sized by 60 mush. 12.5g of Medicago sativa powder, with 0.7% sodium. hydroxide solution as the extraction agent, under the condition of temperature 60 ℃and the ratio of Material to solvent of 1:20(g/v), in a stirring for 20 minutes, was extracted twice and combined filtrate to be used. Second The foam fractionation of Mulberry leaf protein was optimized by response surface methodology. Based on the response surface and contour plots established with protein recovery and enrichment ratio as the response values, the optimum conditions for the separation of Mulberry leaf protein were found to be dilution factor of 40, pH of 5.5, ionic strength of 0.18 mol/kg, and separation temperature of 25 ℃. Under these conditions, the experimental values of protein recovery and enrichment ratio were 92.50% and 7.63, respectively.Second medicago sativa leaf protein as material to Preparated of antioxidant peptides. The Technology was that pH value of 11.6, temperature of 65, alkali protease concentration of 3.0%and time of 4.5. In the condition the clearance ratio was 65.31%.Then the sample flask which had Antioxidant Peptide and 16%trichloroacetic acid was immersed in a constant temperature bath for 10 minute. Sample was centrifugal separationin 5000r/min for 30 minute, then Supernatant was kept,acidolysissand detected. we found it had Asp, Gly, His, Tyr, Val, Leu, PHe, Trp and Lys.