The Research Of The Enzyme Produced By The Kenaf Degumming Strains

Kenaf is applied to the bast fiber textile industry as the important raw materials, which has a long history. There is a great potential market in production of environmental protection of natural fiber by kenaf. Kenaf, as one kind of the 21 st century futuristic crops[1], is farmed massively, which is about 3-5times the tree. At present, the domestic fiber degumming still uses the traditional warm water retting method. The disadvantage of this method is that, the cycle of degumming is long, the higher energy consumption, more water consumption and the more serious environmental pollution. However, it is still stay in the laboratory stage with the enzymatic or bacteria degumming of kenaf, which also cannot undertatake in the large-scale factory application.The microbial strain of kenaf degumming was isolated and identified from the ground soil in our laboratory, which belongs to the famlily Paenibacillus by the 16 s rDNA and named kenaf degumming strain 11-2. The residual gum content of kenaf reaches 10 %-15 % after degumming by the strain. The result shows that the strain could be applied to degumming process of kenaf fiber, which has the high degmming rate to the kenaf.The research is focused on the process of kenaf degumming by the biological enzyme. The main research content is as follow:(1) Sugars was determined as the product after kanaf degumming, according to the measurement of reducing sugar and total sugar content between the control and the treatment using the fermentation liquid of the strain 11-2;(2) The products after kenaf degumming were identified by TLC plate method, which are galacturonic acid oligosaccharides and oligosaccharides;(3) By comparing the degumming producte between the treatment with the strain 11-2 and the control with commercial pectinase, the result shows the fermentation liquid of the strain 11-2 has the higher activity to the kenaf degumming;(4) The two protein were isolated and identified by the ammonium sulphate precipitation and activity of the kenaf degumming;(5) The sequences of two proteins were analyzed by the mass spectrometry, which are pectin lyase. The two proteins were cloned and expressed, which have the activity of kenaf degumming.