Preparation And Application Of Magnetic/Fluorescent Polymer Drug Carrier Microspheres

Magnetic-fluorescent composite nanomaterials is a multifunctional nanoparticles that can integrates magnetism with fluorescence. It can make rapid response to the external magnetic field, and complete the magnetic targeting migration. Furthermore, magnetic-fluorescent composite nanomaterials, possessing the good fluorescence properties, which can be applied to a fluorescent probe for a variety of biological molecules marking and tracing, can be widely used in the biological, chemical, medical and other cross-science fields. Such as fluorescent labels and image, separation and detection of large biological molecules, drug deliveries and cancer therapy.Sodium Demethylcantharidate(SNCID) is a synthetic derivative of cantharidin of Chinese medicine Spanish Fly’s active ingredients, which commonly used in clinical treatment of lung cancer and other cancers. Because of the renal toxicity and short biological half-life of SNCID, it rapidly eliminated in human body through oral or intravenous administration. Thus, to some degree, that restrict its clinical application. Due to this, people ignore the fact that decreasing its toxic side effects and improve the treatment outcome is a hot research topic at present.Since silicon dioxide act as the maskant, combing Magnetic fluoreseent composite nanopartieles with Sodium Demethylcantharidate into magnetic/fluorescent Polymer drug carrier microspheres that can reduce Sodium Demethylcantharidate exposure to the digestive enzymes such as pepsin, avoiding drug decomposition and thus improving the stability of drug in gastrointestinal tract. Furthermore, under the action of externally applied magnetic fields, the drug delivery systems can be targeted to the lesion location to enhance the therapeutic efficacy of the medication, reduce the toxic side effects of high concentrations of drugs on normal tissue. Fluorescence Photography Techniques can monitor the whole process of medicine transporting.1. Magnetic Nanoparticles(MNPs) were prepared with co-precipitation method, Cd Te was synthesized by hydrothermal method, and analyze theirs physicochemical properties. The results shows that MNPs have superparamagnetic and fluidity; QDs have high fluorescence intensity and good stability.2. Amino-magnetic-fluorescent composite nanomaterials(MQDs/Si O2-NH2) were prepared by Reversed-phase microemulsion method, and reacted with the carboxyl of SNCID, make Magnetic/fluorescent Polymer drug carrier microspheres. By studying the fluorescent property, stability, size, magnetic property and dispersion characterizatioin of the composite nanomaterials, the results shows that this nanoparticles are globular. Its particle size is within 45-75 nm, and exhibit the property of fine monodisperse, strong fluorescence and good magnet.3. To research magnetic-fluorescent composite nanomaterials acting as drug loading, average incorporation efficiency of SNCID and its drug release property in static magnetic field.The experimental result indicates that the drug loading and encapsulation efficiency in the microspheres are 10.84% and 70.5%, respectively. Thereby, it can releases drug under the action of static magnetic field, to achieve controlled-release of medicine.4. To introduce the application of Magnetic/fluorescent Polymer drug carrier microspheres in Hep G2 cell markers and the treatment. The experiment results illustrates that 8h incubation, Drug carrier microspheres shows excellent fluorescence label effect. As time pass by, the morphological of the Hep G2 cells of SNCID group and DOX group change gradually. And after 8h, a large number of cells morphological changed, and detached from the cell plate. While, MQDs/Si O2 group, MQDs/Si O2-NH2-SNCID group and MQDs/Si O2-NH2-DOX group have no change in cell morphology Hep G2.Therefore, magnetic/fluorescent polymer drug carrier microspheres possess good properties in magnetic, fluorescent, small size, drug loading, water solubility and stability. It can release drug under the action of static magnetic field, achieving controlled-release of medicine.Its remarkable fluorescent label effect proved in the application of Hep G2 cell.