Toxic Studies Effects Of Cadmium Chloride And Potassium Dichromate On Different Ploidy Loach Fin Cell Lines

In order to explore the mechanism of toxicity, compare the resistance differences between different ploidy cells and find the best test system, this study did a research on the toxic effects of cadmium chloride and potassium dichromate on different cell lines, which used 60-80 generation of diploid, triploid and aneuploid loach fin cell lines(DIMF, TRMF and TEMF) as masterials. The semi-lethal concentrations of cadmium chloride on three cell lines were 24.0±0.7μmol/L, 27.3±1.3μmol/L and 26.7±1.5μmol/L, respectively. The sensitivity of DIMF was the highest. With the increase of the cadmium chloride, Superoxide Dismutase SOD and Glutathione peroxidase GSH-Px of three cell lines increased firstly and then decreased, while Glutathione S-transferase GST decreased gradually. The changes of enzyme activity shown that the resistances of TRMF and TEMF were both higher than DIMF.Micronucleus rates of three cell lines firstly raised, then decreased under the stress of cadmium chloride. And the highest micronucleus rates were 7.33±0.33‰, 8.33±0.67‰ and 9.00±0.01‰ respectively.The metallothionein(MT) expression quantity of three cell lines increased gradually with the concentration of cadmium chloride increased from 0-30μmol/L, and the MT expression quantity was the highest at 30μmol/L which were 55.6, 57.6 and 60.1 fold of thhose in control group respectively. The three cell lines 24h-IC50 were 25.3±1.2μmol/L, 27.9±0.6μmol/L, 28.5±0.4μmol/L when they were treated by potassium dichromate. Low concentration of potassium dichromate could elevate the activities of SOD and GSH-Px, but high concentration of potassium dichromate decreased the activities. The activity of GST decreased with the increasing concentration. The results showed that DIMF’s resistance was very low. The micronucleus rates increased with the increase concentration of potassium dichromate firstly, then gradually decreased.The maximum micronucleus rates of three cell lines were 7.33±0.67‰, 8.00±0.57‰, 9.00±0.67‰. Under the same concentration of potassium dichromate, the order of micronucleus rates was DIMF<TRMF<TEMF. The expression of MT increased significantly after induced by potassium chromate, but the differences between three cell lines were not significant.