4-Phosphatephenyl-Modified Glassy Carbon Electrode For Real-Time And Simultaneous Electrochemical Monitoring Of Paracetamol And Diclofenac In-Vitro Release

Nowadays, the electrochemical detection method has been widely used in continuous(industrial and environmental) monitoring applications. Compared with other drug analytic methods, the electrochemical test has excellent characteristics such as high sensitivity,simple operation, and rapid response. Chemically modified electrode(CME) plays an important role in food, environmental, biological and pharmaceutical analysis. This thesis aims at further application of CMEs in electroanalytical area: the modified electrodes were prepared and used to monitor in-vitro dissolution process of drug-loaded nanofiber system.The developing background of drug-loaded electrospun nanofibers system and the significance of the topic were introduced. In this thesis, a 4-phosphatephenyl(4-PP)covalently modified GCE was used to monitor release kinetics of paracetamol(PCT)or/and diclofenac sodium(DCF) from the electrospun nanofibers. First of all, the calibration curves of PCT or/and DCF on the 4-PP-modified GCE were obtained in a phosphate buffer solution(PBS, pH=7.4, 36.8 °C), respectively. Then, based on the calibration curves, the release processes of the drugs from the electrospun nanofibers were electrochemically monitored in real time. As demonstrated from the experiment results, the4-PP-modified GCE presents advantages of stability, easy fabrication and sensitivity which can be used as a real-time electrochemical platform to monitor in vitro dissolution process of a drug delivery system. The release kinetics study reveals that the drug follows first order kinetics and the mechanism of drug release was diffusion controlled type. Such direct and continuous monitoring capability of the drug release kinetics from drug-loaded electrospun nanofibers offered considerable promise for characterizing the release properties of new drug delivery with optimal drug release properties.