Study On The Synergistic Antibacterial Effect Of Silver-carried Layered A Series Of Zirconium Phosphonate

Bacterial infections are a constant threat to public health, and have led a multibillion dollar burden to the health care system. Although antibiotics have been widely studied and used, the bacteria can become resistant to antibiotics during the long-term use. To address this issue, people began to turn their attention to study a promising inorganic antibacterial agent(Ag NPs). However, free Ag-NPs are highly cytotoxic to mammalian cells, and agglomerated easily in practical application, which thereby limited their application and reduced the antibacterial capacity. In order to overcome these shortcomings,We synthesized a series of zirconium phosphonates and silver carried zirconium phosphonates. We carried out the works as follows:(1) A series of zirconium alkyl-N, N-dimethylenephosphonate silver-carrying(Ag-ZRDP) were successfully prepared and their potential applications as synergistic antibacterial materials were investigated. Silver nanoparticles(Ag NPs), of 30 nm in diameter, were tightly anchored onto the zirconium alkyl-N,N-dimethylenephosphonate(ZRDP), increasing the antibacterial activity of the Ag NPs due to a decrease in the extent of nanoparticle aggregation. Due to the synergistic antibacterial effect of the Ag NPs and ZRDP, the Ag-ZRDP showed a better antibacterial activity than Ag NPs and ZRDP with a minimal inhibition concentration(MIC) of 0.25 and 0.25 μg mL-1against Escherichia coil and Staphylococcus aureus,respectively. Additionally, the Ag-ZRDP did not show obvious cytotoxicity against mammalian cells(A549 cells), even at a concentration of 256 μg mL-1. Collectively,these properties make the newly synthesized Ag-ZRDP potentially superior asdisinfectants and antiseptics for various biomedical and biotechnological applications.(2) Silver-carried zirconium glycine-N, N-dimethylenephosphonate(Ag-ZGDMP)has been successfully prepared. Silver nanoparticles(Ag NPs), of 16-20 nm in diameter,were tightly immobilized onto the zirconium glycine-N, N-dimethylenephosphonate interlayer, and the problem of Ag NPs aggregation and cytotoxicity are solved. The Ag-ZGDMP possessed excellent antibacterial ability against Gram-positive and Gram-negative bacteria of S. aureus and E. coli, due to the synergetic antibacterial activity between ZGDMP and Ag NPs. The excellent performance of ZGDMP and Ag NPs are combined. Ag-ZGDMP possessed long-lasting effects, strong antibacterial properties and better heat resistance, meanwhile, Ag-ZGDMP did not show obvious cytotoxicity against mammalian cells(A549 cells). Therefore, Ag-ZGDMP is an efficient and non-cytotoxic synergetic antibacterial agent, deserving further application.(3) In this work, the success of Ag-ZRDP and the polylactic acid compound,preparation of the antibacterial function of polymer composite materials.Through mechanical properties characterization indicates that add a small amount of silver zirconium organic phosphonic acid on the mechanical properties of poly(lactic acid)(PLA).By silver antibacterial experiments show that zirconium organic phosphonic acid and polylactic acid composite material has good antibacterial properties.Show that organic phosphonic acid silver as antibacterial material can be applied to polymer and have good antibacterial effect.