Oscillating Magnetic Field Design About The Separation Of Immune Complexs

Chemiluminescence immunoassay analyzer is one instrument of usingchemiluminescent principle to detect diseases, which is widely used in the diagnosis ofhepatitis, cancer and other diseases. Due to the magnetic force, immune complexes areabsorbed to the both sides of the cuvette stacked together, forming large agglomeratesand wrapping up the uncombined makers in the fixed magnetic separation process of theanalyzer. The makers cannot be cleaned effectively in washing process, resulting inlarger test results. Reunion immune complexes can vibrate in an oscillating magneticfield with the magnetic field. To this end, the research on variable magnetic separationto weaken aggregate aggregation, releasing the uncombined markers is meaningful forimproving the washing effect and the detection accuracy.Firstly, the theory of the immune magnetic separation is introduced in the paper,and mechanical model of the magnetic particles is built based on magnetic fluid andother related theories with a detailed analysis. Shear yield stress model of magneticparticles chain is built to analyze the suffered shear stress. The oscillation condition ofthe chains of particles is analyzed, and the oscillation parameters design principles ofthe permanent magnet is determined to provide theoretical support for the follow-updetermine of the magnetic field parameters.Secondly, the theory and modeling method of magnetic field simulation areconducted in this paper. Modeling and simulation containing2D and3D magnetic fieldof the cylindrical permanent magnets used in the analyzer are carried out, using themethod of magnetic field simulation to determine the critical point of the magnetic fieldgradient changing direction which can cause magnetic particles to oscillate. Themagnetic field simulation has been verified by the comparison between the simulationand true value. Vector contours of magnetic induction2mm away from the end face ofthe permanent magnet is achieved by importing magnetic flux density data file intoMATLAB for processing. Through analysis of the cloud, the minimum amplitude ofmagnetic oscillation is given. Magnetic particle motion dynamics model is established,which is analyzed to determine the optimum oscillation frequency of the magnet.On this basis, a variable magnetic separation experimental observation platform isconstructed in this paper, using SCM to control stepper motor driving cam operating fortarget amplitude and oscillation frequency. Experimental observation of macroscopicagglomerates is performed, finding that both sides of the accumulation of magneticparticles spread to the center which weakens agglomeration of magnetic particles. Useinverted biological microscope to perform a microscopic observations, finding that the accumulation of magnetic particles chain spreads more uniform under oscillatingmagnetic field.Finally, magnetic particles are isolated by manual blowing, the static andoscillating magnetic field separation. Compounds obtained by the three separationmethods of the above are detected for the light-emitting values to make a comparativevalidation by the chemiluminescence immunoassay analyzer, finding that alternatingmagnetic separation method can effectively weaken aggregate wrapping effect to theuncombined markers.