Laser Technology Of Microlens Solidified On Needing Positions Dynamically With Research About The Non-spherical Characteristics

Harmful microbes in spacecraft harm health of astronauts and corrode thespaceflight’s materials, so it is urgent to develop real-time and automatic alarmsystem of space microbes. Kernel of the system is fluorescence detectionmicro-system. General photo-devices can’t be inserted into biochip due to its bigvolume. Special devices can be inserted into the biochip due to the smaller volume,but its sensitivity can’t meet the demands. It is the bottleneck of the biochip whichmany nations want to solve urgently at present.According to the bottleneck problem, there is a laser manufacture technologyintroduced in the thesis. The technology is based on the phenomenon that itsnon-sphere shape of top face can change two times alternatively when optic glue dripis dropped on base face freely and its surface shape is in process from impactingmodification to resuming stable infiltration state. The manufacture process of thetechnology is as follows. Shape of the optic glue drip with nano-quartz particles issnapped dynamically in practical micro-workface. The glue is irradiated by ultravioletlaser at due time and it is solidified to form optic micro-lens with non-sphere top facefitting designing shape. The non-spherical shape of the lens made by the technologycan increase the acquisition amount of the photons in the fluorescence detectionmicro-system. As the coaxial adhesion between the micro-lens and the workface highaccurately can increase focusing efficiency. And the optical cement doped withnano-quartz particles can improve mechanical performance and smoothness of themicro-lens. The biochip bottleneck problem can be solved by the perfect opticperformance of this original position intensification micro-system.Contents of the thesis include laser technology of manufacturing micro-lenssolidified in situ dynamically, achieve the specific implementation details of thetechnology and the research about the characteristics of the optical micro-lenssolidified in situ dynamically. According to the contents above, the related researchwas launched and the conclusion was achieved as follows:(national invention patentrelated the technology has been be licensed)2. According to the micro-manufacturing laser technology for three-dimensionalmicro-optics systems, the technical characteristic analysis and research are surveyed.The detail contents include: manufacturing non-spherical optical micro-lens solidifieddirectly on the plane of excitation units and detection units in the fluorescencedetection system required for optical micro-lens. This method doesn’t need anyassistive tool in micro-scale, such as a mold or a mask and so on. And it avoids thetwo technical problems by using the traditional methods. At the same time, the size ofthe optical micro-lens, which is made by the technology and verified by theexperience, can reach the scale of millimeter magnitude, even micrometers. It isbenefit to make the light focusing effect more perfect and improve the detectionsensitivity by using the non-spherical micro-lens. Ultimately it makes micro-spectraldetection system microminiaturized. The system can be embedded bio-chip. And its high sensitivity is able to meet the requirements of biotechnology to detect weaksignals. Thereby it guarantees the successful completion of the whole process ofanalysis and inspection procedures.3. According to the characteristics of the optical micro-lens solidified in situdynamically, the research is verified in both theoretical and experimental aspects ofanalysis and argumentation. The following contents are the specific description.(1) Theoretical studies. Based on the hydrodynamic mechanism theory, which isinvolved in the laser technology of manufacturing micro-lens solidified, and thephenomenon of the optical cement droplet spreads around on the horizontal plane dueto the surface tension, the mechanics analysis is developed on an any point on surfacefor observation. The surface equations representing the relationship of all thecoordinates point was built. And equation sets of the surface were solved combinedwith numerical methods. By setting the different performance parameters of thecement droplet, such as contact angles and characteristic length, droplet surface shapeparameters were calculated. And based on the calculation results, the curve of dropletssurface shape and radius of curvature are described by the result data. The influenceparameters of non-aspheric lens cement droplet were achieved.(2) Experimental studies. There are the experiments with the relationshipsbetween the surface roughness of the substrate and contact angle of NOA61cementdroplet, the experiments with the relationships between doping concentrations of theNOA61cement droplet and contact angle and the sensitivity detection experiments inthe miniature real-time fluorescence quantitative PCR detection system.Both of thetheoretical calculations and experimental verification showed that the micro-lensmade by using optical cement doped impurities and polishing substrate have the bestnon-spherical characteristics. It improves the sensitivity of the system and meets thetechnical requirements for biological fluorescence PCR detection at the same time.