Isolation And Identification Of The Protiens Interacting With 14-3-3 Proteins In Laticifer Of Hevea Brasiliensis

Laticifers in rubber tree (Hevea brasiliensis), the only commercial source of natural rubber, are the tissues specific for the biosynthesis and storage of natural rubber as well as defense against pathogens. Natural rubber is synthesized and made almost entirely of isoprene units derived from isopentenyl diphosphate (IPP) by a mevalonate (MVA) pathway. Although some gnens controlling natural rubber biosynthesis have been cloned and characterized, the molecular mechanism regulation of natural rubber biosynthesis remains unclear.In plants 14-3-3 proteins have been found to regulate a variety of biological processes such as metabolic, growth and developmental or signaling pathways via interactions with their target proteins. They have been involved in regulation of protein synthesis, protein folding, gene expression, primary metabolism including hormone metabolism, plasma membrane-localized proton pump, organellar/nucleocytoplasmic shuttling, and chromatin remodeling. Several studies have also established a role for 14-3-3 proteins in signaling pathways and environmental stress response. Although the 14-3-3 proteins participating in various signalling pathways have been well characterized in a few plants, little is known so far about how the rubber tree 14-3-3 proteins regulate natural rubber biosynthesis through interacting with target proteins.Through yeast two-hybrid screening,25 interaction partners of the three 14-3-3 proteins (HbGF14a, HbGF14b, and HbGF14c), which are involved in plant development, metabolism, signalling transduction, and other cellular processes, were identified in laticifers. Taking these data together, it is proposed that the 14-3-3 proteins may participate in regulation of natural rubber biosynthesis. Thus, the results of this study provide novel insights into the 14-3-3 signalling related to natural rubber biosynthesis.