| The study about researching the impact of transgenic maize on soil microbe, soil physical and chemical properties is the very significant content in environmental safety evaluation of transgenic maize. In this paper, we study the crylAc transgenic insect-resistant maize and receptor control which were planted in Gongzhuling City of Jilin Province. In my paper, the separation and culture technology of phosphate-solubilizing bacteria, molecular identification technology and activity detection technology of soil enzyme were applied in this paper. During seven different growth periods of maize, we gathered the maize soil samples and studied the impact of the crylAc transgenic insect-resistant maize on the number, the resolving phosphorus ability and the genus distribution of phosphate-solubilizing bacteria. At the same time, we studied the impact of the crylAc transgenic insect-resistant maize on soil urease activity、soil invertase activity and soil dehydrogenase activity.The result showed as below:Compared with the receptor control, the crylAc transgenic insect-resistant maize showed significant influence in the number of phosphate-solubilizing bacteria during different growth periods, some times promoting, some times inhibiting. At the same time, the crylAc transgenic insect-resistant maize showed promoting action in the resolving phosphorus ability of phosphate-solubilizing bacteria in some degree.Compared with the receptor control, the number of soil phosphate-solubilizing bacteria of the crylAc transgenic insect-resistant maize during different growth periods had similar change rules with receptor control. The number of soil phosphate-solubilizing bacteria was much higher than the other 5 stages in heart leaf stage and jointing stage.Compared with the receptor control, the resolving phosphorus ability of the cry1Ac transgenic insect-resistant maize during different growth periods had similar change rules with receptor control. The resolving phosphorus ability of soil phosphate-solubilizing bacteria was much higher than the other 4 stages in heart leaf stage, jointing stage and flare opening stage. The 75 isolates from cry1Ac transgenic insect-resistant maize belonged to 17 genus, and the 89 isolates from receptor control belonged to 14 genus. It meant that the genus distribution of phosphate-solubilizing bacteria was more abundant and complex in the soil planted cry1Ac transgenic insect-resistant maize. Bacillus and Burkholderia were the predominant group in both cry1Ac transgenic insect-resistant maize and receptor control.Compared with the receptor control, the activity of main functional enzyme in soil of the cry1Ac transgenic insect-resistant maize during different growth periods had similar change rules with receptor control. The soil urease and soil invertase showed the lowest activity before sowing seed and then gradually rose. They didn’t get the highest activity until flare opening stage and then moderately declined. The soil dehydrogenase activity showed the lowest activity before sowing seed and then quickly rose to the highest activity in heart leaf stage. The dehydrogenase activity becomes quickly decline in the jointing stage, after that, the downtrend gets more and more slow. Compared with the receptor control, the cry1Ac transgenic insect-resistant maize had no significant impact on soil urease activity and soil invertase activity, but some promoting on soil dehydrogenase activity.In summary, the cry1Ac transgenic insect-resistant maize could impact the diversity of soil phosphate-solubilizing bacteria and activity of soil main functional enzyme in some degree. The population structure of soil phosphate-solubilizing bacteria and the change of siol main functional enzyme in field planted the crylAc transgenic insect-resistant maize had revealed in my paper. The results provided some scientific basis for the evaluation of environmental safety and some theoretical basis for sustained and healthy development of Bt transgenic insect-resistant maize. |
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