| Over the last decade, many works were invested in the research of transgenic Bt-rice. Some rice strains with highly insect resistant properties have been obtained and their way to their commercialization has been stepwisely paved. It is necessary to implement a comprehensive safety evaluation to Bt-rice to avoid potential risks. Determination the key nutrients, amino acids, trace elements and anti-nutritional factors in brown rice of transgenic Bt-rice and its wild type, have been carried out to give an evaluation of the substantial equivalence of transgenic Bt-rice and its parents. Besides, novel and improved analytical methods have established to detect the microelements, heavy metals and tannins in transgenic-Bt rice and wild-type rice.The main contents are described as follows:1. Determination and comparison of main components between Bt transgenic rice and wild-type riceThe key nutritional constituents, mineral elements, heavy metals and anti-nutritional factors in two kinds of transgenic rice with Bacillus thuringiensis gene (A, B) were determined and compared with their wild-type(C) under the substantial equivalence. The results show that no significant difference have found in the content of the key nutritional constituents, mineral elements, heavy metals and anti-nutritional between two transgenic rice and wild-type rice, except the content of Fe, Mn, Cu and Pb have significant difference between two transgenic rice and wild-type rice. It suggested that the transgenic process has not obvious change content of main composition and character of wild-type.2. Study on the determination of microelements in transgenic Bt rice by slurry-sampling combined with FAASA new simple method for rapid determination of potassium, calcium, magnesium, iron, manganese, zinc and copper in brown rice of transgenic Bt rice and wild-type rice by slurry-sampling combined with FAAS. Variables affecting the determination of microelements, such as the vibrate method of the suspensoid, concentration of Agar, the acidity of the suspensoid and detection method, were optimized. Under optimized condition, the recoveries were in the range of 90-106% for detection of microelements in transgenic Bt rice and the wild-type rice samples. Determination of the transgenic brown rice and the wild-type brown rice samples by this method.3. Study on the determination of trace lead and cadmium in transgenic Bt rice by CCMKGM-based micro-column extraction combined with GFAASA novel method was developed for the determination of trace lead and cadmium in transgenic brown rice based on separation and preconcentration with a micro column packed with CCMKGM prior to its determination by graphite furnace atomic absorption spectrometry. Variables affecting the separation and preconcentration of lead and cadmium, such as the acidity of the aqueous solution, sample flow rate and volume, and eluent concentration and volume, were optimized. Under optimized condition, detection limits of the method for the determination of trace lead and cadmium in transgenic brown rice were 0.11μg/L and 0.002μg/L. The recoveries were in the range of 90-103% and 93-105% for detection of Pb and Cd in transgenic rice and the wild-type rice samples respectively. Determination of the transgenic brown rice and the wild-type brown rice samples by this method.4. Study on the determination of tannin in transgenic Bt rice by HPLCA new method was developed for the determination of the tannin in transgenic Bt rice with HPLC after exrraction by organic solvent. The type of extractant, the amount of extractant, extraction methods, and the extraction time and flow rate of mobile phase were studied. Under optimized condition, the detection limit of the method for the determination of tannin in transgenic brown rice was 4.7 ng/mL. The recovery was 103% for detection of tannin in transgenic rice and the wild-type rice samples respectively. Determination the taanin of the transgenic brown rice and the wild-type brown rice samples by this method. |
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