Effects Of Atrazine And Photosynthetic Bacteria On Immunity Of Neocaridina Denticulate Sinensis

In this paper, Neocaridina denticulata sinensis as the experimental material to study thatIncluding the proportion of Semi-granular cell and the activity of superoxide dismutase(SOD), alkaline phosphatase (ALP), acid phosphatase (ACP) of N.denticulata sinensis byatrazine gradient processing, adding Rhodobacter sphaeroides, Bacillus subtilis and mixedbacteria into the water and starvation.The N. denticulata sinensis not only has certaineconomic value, but also because of their advantages such as individual small, growing fast,is a kind of ideal material for environmental monitoring.So the result not only have thetheoretical value, but also practical guiding significance.After acute toxicity experiment of atrazine on the N. denticulata sinensis,96hLC50ofatrazine on the N. denticulata sinensis is29.17mg/L.The proportion of Semi-granular cell of N. denticulata sinensis is38.41%. Experimentalresults show that atrazine can significantly reduce the proportion of Semi-granular cell of N.denticulata sinensis blood cells(P<0.05). As the atrazine concentration and exposure timeincreases, the proportion of Semi-granular cell of the N. denticulata sinensis Blood cellsdecreases gradually.The activity of SOD, ALP and ACP of hepatopancreas, muscle and ovary of N.denticulata sinensis is21.17±0.09U/mg,8.35±0.03U/mL,12.24±0.04U/mL and4.65±0.09U/mg、0.33±0.01U/mL,1.03±0.02U/mL and7.135±0.12U/mg,1.19±0.01U/mL,0.72±0.01U/mL. The activity of SOD, ALP and ACP of Hepatopancreas is the highest and thehepatopancreas is more sensitive to atrazine than muscle and ovarian.It is similar to theinfluence of high concentrations of atrazine on the activity of SOD of hepatopancreas,muscleand ovarian of N. denticulata sinensis.The process was first increased and thendecreased.Low concentrations of atrazine can enhance the activity of SOD ofhepatopancreas,muscle and ovarian of N. denticulata sinensis. As the atrazine concentrationsand exposure time increases, the activity of ALP and ACP of hepatopancreas, muscle andovary decreases gradually. The activity of ALP and ACP hepatopancreas, there aresignificant differences in the concentration and time.After the treatment of mixed bacteria, B. subtilis and R. sphaeroides,there was significant difference in the proportion of Semi-granular cell of the N. denticulata sinensis between experimental groups and the control group (P<0.05).The proportion of Semi-granular cell of the mixed bacteria group, Bacillus subtilis group and Rhodobacter sphaeroides group were respectively8.10%,7.19%and6.75%higher than control group. After starvation, The proportion ofmixed bacteria group, Bacillus subtilis group and Rhodobacter sphaeroides group were respectively26.31%,24.56%and33.74%hgher than control group. The experimental resultsshowed that R. sphaeroides can increase and keep the immunity of N. denticulata sinensis.Under normal feeding conditionsthe activity of SOD, ALP and ACP of Hepatopancreasmixed bacteria group, Bacillus subtilis group and Rhodobacter sphaeroides group were respectively8.10%,7.19%and6.75%;8.22%,3.52%and12.72%;40.12%,12.72%and20.45%hgher than control group. And there was significant differencebetween it and control group (P<0.05). After starvation,there was no significant difference ofenzyme activity in muscle and ovary between it and normal feeding conditions (P>0.05). Inthe blank control group SOD activity increased,ALPand ACP activity decreased.There was significant difference between it and normal group (P<0.05). Mixed group of bacteria, Bacillussubtilis group and Rhodobacter bacteria group hepatopancreas SOD activity were10.23%,6.07%and5.25%lower than the blank control group; The ALP and ACP activity of mixed group of bacteria, Bacillus subtilis group and Rhodobacter bacteria group hepatopancreas were respectively21.20%,17.99%and27.56%higher than the blank control group,;43.24%,21.62%and27.56%.