Apparent Digestibility Of 18 Feed Ingredients For Bluntnose Black Bream Megalobrama Amblycephala Yih

This study was conducted to determine the apparent digestibility of 18 different feedstuffs for bluntnose black bream Megalobrama amblycephala Yih, and compared the differences bewteen two fecal collection methods, in order to providing theoretical basis for optimizing the feed formular for bluntnose black bream, which included there series:Serie 1 Apparent digestibility of nine conventional feed ingredients for bluntnose black bream Megalobrama amblycephala YihThe apparent digestibility (AD) for dry matter, protein, crude fat, amino acids, phosphorus and energy of nine feed ingredients for bluntnose black bream were determined by using 0.5%Cr2O3 as an indicator. The test diets consisted of 70% reference diet and 30% test feed ingredients including fish meal, meat and bone meal, soybean meal, peanut meal, cottonseed meal, rapeseed meal, DDGS, wheat middling and rice bran. The fish averaging (296.54±1.71)g were reared in indoor aquarium tank (3.0m×0.8m×0.8m), the fecal samples were collected by abdominal pressure method after one week. The results showed that apparent digestibility for dry matter, protein, crude fat, amino acids, phosphorus and energy of nine feed ingredients were 45.10%-77.48%,56.78%-93.63%,62.36%-95.80%, 62.49%-94.83%,3.21%-48.02% and 49.42%-85.80% respectively. In the nine feed ingredients, the highest apparent digestibility for dry matter protein and total amino acids was rapeseed meal; the apparent digestibility of fish meal was closed to peanut meal and soybean meal; and the lowest was wheat middling. DDGS showed the highest apparent digestibility for crude fat, the following were rice bran and fish meal, and the lowest were peanut meal and rapeseed meal. Apparent digestibility of phosphorus were lower in addition to DDGS, the lowest were rapeseed meal and meat and bone meal, and others were 9.13%-29.10%. Energy apparent digestibility of rapeseed meal was consistant with fish meal and meat and bone meal. Serie 2 Apparent digestibility of nine unconventional feed ingredients for bluntnose black bream Megalobrama amblycephala YihThe apparent digestibility(AD) for dry matter, protein, crude fat, amino acids, phosphorus and energy of nine feed ingredients for bluntnose black bream Megalobrama amblycephala Yih were determined by using 0.5%Cr2O3 as an indicator. The test diets consisted of 70% reference diet and 30% test feed ingredients including expanded feather meal, enzyme feather meal, blood meal, silkworm pupa, corn gluten meal, peptide, broken rice, corn, and barley grain. The fish averaging (250.83±2.12)g were reared in indoor aquarium tank(3.0m×0.8m×0.8m), fecal samples were collected by abdominal pressure method after one week. The results showed that apparent digestibility for dry matter, protein, crude fat, amino acids, phosphorus and energy of nine feed ingredients were 54.46%-92.69%,81.54%-92.75%,62.28%～103.44%,63.07%-95.56%,41.48%～97.55% and 59.89%-97.81%. Corn gluten meal showed the best apparent digestibility for all nutritions, and peptide had the lowest digestibility for dry matter, crude fat and energy. Which had the highest apparent digestibility of four animal protein feedstuffs was silkworm pupa, and next was blood meal. Apparent digestibility of enzyme feather meal was better than expanded feather meal in addition to crude fat. Barley grain had the highest protein and amino acid digestibility, but the apparent digestibility for dry matter, crude fat and energy were significantly lower than that of corn and broken rice.Serie 3 Reserch of apparent digestibility of ingredients for bluntnose black bream Megalobrama amblycephala Yih by two methods of fecal collectionThe apparent digestibility(AD) for dry matter, protein, crude fat, amino acids and phosphorus of nine feed ingredients for bluntnose black bream Megalobrama amblycephala Yih were determined by using 0.5%Cr2O3 as an indicator. The test diets consisted of 70% reference diet and 30% test feed ingredients including expanded feather meal, enzyme feather meal, blood meal, silkworm pupa, corn gluten meal, peptide, broken rice, corn and barley grain. The fish averaging (250.83±2.12)g were reared in indoor aquarium tank (3.0m×O.8m×0.8m), the fecal samples were collected by abdominal pressure method after one week until enough, and then collected fecal samples from section of intestinal by dissection. The results showed that apparent digestibility for dry matter, protein, crude fat, amino acids and phosphorus of feed ingredients by abdominal pressure method were higher than dissection for bluntnose black bream. However, the apparent digestibility for protein and amino acids were had no significant differences between two methods compared with dry matter, crude fat and phosphorus.In conclusion, rapeseed meal, soybean meal and peanut meal can be used as high quality of plant protein source to alternative part of fishmeal for bluntnose black bream in the actual production. Rice bran was a kind of high quality energy feed ingredient because of higher energy. The apparent digestibility of protein of DDGS was not only higher than meat and bone meal, but the apparent digestibility of phosphorus was highest of nine feed ingredients. Therefore, DDGS can be used as a high quality fish feed ingredient, corn gluten meal and silkworm pupa can be used as high quality plant protein and animal protein resource, respectively. Blood meal, feather meal can be used as a small amount with other feedstuffs. Corn and broken rice can be used as the main energy feed ingredients because of the higher energy values, and barley grain also can be used suitably for bluntnose black bream because of the balance of amino acids. In addition, it was suitably to determine the apparent digestibility for dry matter, crude fat and phosphorus by abdominal pressure method, and protein and amino acids by dissection.