Characteristics Of Growth Traits Of The Mud Crab (scylla Paramamosain) And Identification Of Their Associated Microsatellite Markers

Because of fast growth rate, large culture scale, and significant commercial values,the mud crab (Scylla paramamosain) is one of the important economic crabs, With theincrease of the demand of the market, amount of farming and fishing is increasing yearby year. However,the germplasm resources of the mud crab is declining by deteriorationof the environment, High density cultivation mode, overfishing.Under this background,the artificial breeding and construct the advantage based group become more and moreimportant. In this study, we measured and analysized the correlation between thegrowth trains. we found the growth trains which is different in females and males. Atlast, Build the optimal linear regression equation in females and males.129microsatellite markers and there growth were analysized which is sequencing-basedtranscription. Aims to build and genetic breeding of the mud crab (Scyllaparamamosain) group to carry out basic advantages provide a theoretical basis. Theresults are as follows:1.96individuals were selected in randomly in the G1of the mud crab (Scyllaparamamosain). gender of96individuals was identified, and16growth trains weremeasured. After recording proportion of male and female,the differeces between femaleand male in16growth trains, the correlative in the16growth train in famale and male,impact of15growth trains to body weight were analysized with the SPSS. The ratio offemale and male is1:2. nine growth trains were found which is signifcant differencebetween female and male. In female, the correlation coeffcient of16growth trains isbetween0.524-0.997. In females, a trait arapace length and distance between lateralspine2on body weight have a significant direct effect, the path coefficients were1.124and-0.186. The determination coefficient of arapace length and distance between lateral spine2to the body weight were1.263and0.035, the composite index was0.951,suggesting that these two traits is the main independent variables in affecting bodyweight in female individuals. In male, the correlation coeffcient of16growth trains isbetween0.881-0.999. body height, fixed finger height of the claw and meropodit lengthof peraeopod2on body weight have a significant direct effect, the path coefficientswere0.484,0.300and0.225, and the determination coefficient were0.234,0.090and0.051. The composite index was0.967, suggesting that these three traits is the mainindependent variables in affecting body weight in male. At last we constructed twooptimal linear regression equation, female: Y (BW)=4.969X1(CL)–0.758X2(DLS2)–140.177, male: Y (BW)=3.806X1(BH)+2.371X2(FFHC)+1.725X3(MLP2)–123.559.2.30polymorphic microsatallite markers were found in G1of the mud crab(Scylla paramamosain),which is in129microsatallite markers. observed heterozygosity,expected heterozygosity of the polymorphic microsatallite markers was0.19-1.00and0.23-0.96. PIC of the microsatallite markers was0.21-0.95. Analysis of microsatallitemarkers associated with16growth traits was taken in SPSS. The results show that threemicrosatellite markers were associated with12growth traits. Scpa36was associatedwith CL, carapace length, AW, abdomen width, BH, body height, FFLC, fixed fingerlength of the claw. FFWC, fixed finger width of the claw, FFHC, fixed finger height ofthe claw, MLP2, meropodit length of peraeopod2, MLP3, meropodit length ofperaeopod3. Scpa75was associated with ICW, internal carapace width, AW, abdomenwidth, CWS8, carapace width at spine8, DLS2, distance between lateral spine2, MLP3,meropodit length of peraeopod3. Spm30was associated with BH, body height, DLS2,distance between lateral spine2, BW, body weight. Some genotypes (Scpa36BC,Scpa75BC and BD, Spm30AC) showed the best genotyp in some growth traits in themud crab (Scylla paramamosain).