Morphological Observation Of AIV H5N5Subtype Isolated From Duck And Sequencing Of Its Nucleoprotein And Matrix Protein Gene

Avian influenza is caused by Orthomyxoviridae influenza A virus whichcan infect poultry diseases. It consists of8different RNA segments. The virus can causechicken, Turkey and other poultry and waterfowl, birds death. It also can infect human. The AIVhas many subtypes. The subtype is based on emagglutinin (NP) and neuraminidase (M). The HAhas16subtypes and the NA has9subtypes. With different combination of HA and NA willform different subtypes AIV. Most of them have no pathogenicity or only lower pathogenicity.A little of them have strong pathogenicity. The H5N1,H9N2,H7N7subtype can infect thehuman. Experiments showed that some lower pathogenicity can not infect human, but it canchange power pathogenicity when it pass a period of time in the chicken, ducks and otherpoultry.The AIV has a wide distribution in the world. It has found in America Europe and othercountries and regions now. After it first found in China at1995, it will be isolated in each yearand it showed a normal trend.This study observed it by electronic telescope and found that the virus had theclassical morphology of orthomyxovirus and budded from the cell membrane. Accordingto influenza virus sequence published in NCBI we design two pairs of primers for M gene andNP gene. The PCR products were purified and clone into pMD-18T. The plasmid wastransformed into E.coli DH5α. Extraction of plasmid and sequenced to bio-technologyCompany. The sequencing results were analyzed, sequence analysis and protein molecularcharacteristics analysis, prediction and analysis of signal peptide, Tran membranehydrophobic analysis, potential glycosylation and phosphorylation sites. We will provide atheoretical basis for the prevention and control of avian influenza virus.