Comparative Study Between Biological Characteristics Of The Different Animal Species Aeromonas Veronii And Four Virulence Genes

Aeromonas veronii as a human-animal-fish were suffering from pathogens, could notonly cause disease of aquatic organism such as shrimp, channa argus and crucian as soon, butalso human and mammal suffering from various diseases. Aeromonas veronii produce largenumber of virulence factor and has a close relationship with the disease.The thesis takes different animal derived Aeromonas veronii as investigative object.Bycomparing eight different veronii gas unit cells’ characteristic come from various sourcesnamely from dyeing properties physicochemical properties, cultural traits, different factors(various PH value,temperature, permeation pressure) is influence to its growth,drug toleranceand16s universal primers for comprehensive analysis of the similarity of its other aspects in apreliminary understanding of different endogenous Aeromonas veronii biological characteristicsin common and differences. The results showed that: Aeromonas veronii of the stainingcharacteristics are Gram-negative short bacillus, showed a high degree of consistency in thephysical and chemical properties, and not be tolerated in different strains of high osmoticpressure, low PH.At the present time, the systematicness of research to Aeromonas veronii virulence factor islimited.The thesis designs four pairs of specific primers to amplify Aeromonas veronii fromvarious animal origin. Most strains amplified fragment. It has gone through comparativeanalysis of gene sequences announced by GenBank. It shong that its homology has reachedashigh as100%at least abore77%. It could be showed that different animal original Aeromonasveronii remains highest conservation by an analysis in conservation functional domain by NCBIa online software. It makes up phylogenetic tree for researching its genetic evolution, and provesthat there is similarity among genetic relationship of different source. Four kinds of virulencegenes of different animal original Aeromonas veronii’s comparative research can offerdeveloped genetic engineering vaccine the corresponding in basis of the theoretical.