The Research Of Intestinal Microbial In Hypophthalmichthys Molitrix And Aristichthys Nobilis In Three Different Environment

Fish intestinal microbes play the irreplaceable role on food digestion, nutrientabsorption, immunity and growth to the host. Fish intestinal microbes is an importantpart of fish intestinal. Different kinds、quantity of fish intestinal microbes and thecomplex intestinal microbial community structure guarantee the security of fishnutrition absorption, intestinal immunity and intestinal physiological regulation.Research on fish intestinal bacteria show physiological characteristics of pathogenicmicroorganism and is the guidance of fish diseases; Some intestinal microbes can beused as biological indicator of environmental pollution and microbial indicator in thedetection of water environment changes; Some intestinal microbes can help thedevelopment of micro ecological preparation and provide theoretical basis for feedadditives. Fish intestine contains a large number of microorganisms, forming a naturalgermplasm resources and these microorganisms mostly can not be cultured. Study thefish intestinal microorganisms plays an important role in microbial engineering.Because the traditional microbial purification and cultivation method can only beused in the culturable intestinal microbial, this way has strong personal subjectivity inbacterial colony morphology, colony color change and intestinal microflora of most insurvival are not culturable. Therefore, the traditional method have great deficiency instudy of fish intestinal microbes. The rapid development of modern molecular biology,the molecular biology methods are being widely applied to microbial study.This article uses the PCR-DGGE technique to analyse intestinal microbialcommunity structure and diversity of Hypophthalmichthys molitrix in different polyculture model. PCR-DGGE fingerprinting shows the band numbers ofHypophthalmichthys molitrix single mode for each sample are14、13、14and theaverage band number is13.3; the band number of Hypophthalmichthys molitrixpolyculture mode for each sample are13、16、15and the average number of bands is14.7. UPGMA cluster analysis and similarity analysis of PCR-DGGE fingerprint bandsshow that the average similarity coefficient of Hypophthalmichthys molitrix singlemode samples is0.694; average similarity coefficient of Hypophthalmichthys molitrixpolyculture mode sample is0.639. The sequencing results show the main intestinalmicrobials in Hypophthalmichthys molitrix are Bacteroidetes、Firmicutes、Proteobacteria、Verrucomicrobia and Cyanobacteria, and the Proteobacteria is in thepredominance.This article uses the PCR-DGGE technique to analyse intestinal microbialcommunity structure and diversity of Hypophthalmichthys molitrix and Aristichthysnobilis. PCR-DGGE fingerprinting shows the band numbers of Hypophthalmichthysmolitrix for each sample are18,18,12and the average band number is16, illustratingsmall differences between individuals; the band number of Aristichthys nobilis for eachsample are17,12,13and the average number of bands is14, showing large differencesbetween individuals. UPGMA cluster analysis and similarity analysis of PCR-DGGEfingerprint bands show that the average similarity coefficient of Hypophthalmichthysmolitrix samples is0.569and at a medium similar level; average similarity coefficientof Aristichthys nobilis sample is0.623and also at a medium similar level. Thesequencing results show the main intestinal microbials in Hypophthalmichthys molitrixare Bacteroidetes, Firmicutes, Proteobacteria and Verrucomicrobia; the main intestinalmicrobials in Aristichthys nobilis are Bacteroidetes, Proteobacteria, Cyanobacteria andFirmicutes.. This article uses the PCR-DGGE technique to analyse intestinal microbial ofHypophthalmichthys molitrix and Aristichthys nobilis in complex ecological farmingsystem. PCR-DGGE fingerprinting shows the band numbers of Hypophthalmichthys molitrix for each sample are15,17,15and the average band number is15.3; the bandnumber of Aristichthys nobilis for each sample are19,13,11and the average number ofbands is13.3. UPGMA cluster analysis and similarity analysis of PCR-DGGEfingerprint bands show that the average similarity coefficient of Hypophthalmichthysmolitrix samples is0.621; average similarity coefficient of Aristichthys nobilis sampleis0.636. The band numbers of Hypophthalmichthys molitrix is higher than Aristichthysnobilis, and has a lower similarity, show that the Hypophthalmichthys molitrix has amore complex intestinal microbial community structure and diversity.