| As a widely distributed around the world, marbled eel, due to its breedingperformance, high nutritional value, delicious taste, in recent years has become widelyaccepted as a special object of aquaculture. But Anguilla’s amazing migratory lifehistory and reproductive complex reproductive regulation makes it difficult to breakthrough artificial breeding, which has been to rely on the annual offspring tide,fishermen catch glass eels newborn as seed supplement from nature. Coupled with theecological destruction and global climate change of the environment, eel resources arediminishing every year, which greatly limits the eel aquaculture industry developmentand species protection activities. Eel fry prices affect the entire lifeline eel industry,which makes artificial breeding eel research has become particularly urgent andcritical.This study focus on marbled eel that is national animal protection, based onartificial propagation latest research both at home and abroad, carried out the artificialreproduction of marbled eel spawning experiments, we designed to optimize artificialinduction for gonads mature technology and a preliminary inquiry artificial inductionof gonadal development process of eel’s endocrine physiology conditions.Mainly studied from the following four aspects:1, For low egg quality uneven during gonad development in the ripening processof artificial reproduction of eel, combining recent research results domestically andabroad, indicating that the male hormone generates a significant role in promotingeffect for eel ovarian development early yolk, we design different hormones toinduced gonadal development marbled eel gonads. The results show that: the use ofCPE and HCG (group B) can effectively induce marbled eel gonadal maturation, withincreasing induction, sampling averaged22.9%, induction effect is remarkable. The A,C, D groups with little effect induced by hormone injections, GSI is1%-3%.Particular group or C gonads showed degradation of only0.5%. By using the results of that marbled eel ADSD did not induce ripening effect,2, We chose mature females eel of group B and segment induced mature males,making the male and female fish reach maturity together, optimized for marbled eelbreeding technology, access to a large number of high-quality sperm. Then for maleand female fish were spawning eels, but did not get a successful hatch seedlings. Onthis basis, we observe egg morphology and review the whole experimental process,lessons learned on how to grasp the opportunity of late stage of maturation preciselywere discussed and summarized, the conclusion is there are some differencesbetween marbled eel and Japanese eel, just by weight and shape observation is easy tomiss the best opportunity for late stage of maturation, resulting in the induction ofpremature or over matured egg, these research provides the first practical informationto artificial propagation followed by those working in the marbled eel.3, In addition using real-time fluorescence quantitative pcr technology, inducedpituitary gonadotropin subunits different period: GtHα, LHβ, and FSHβ were studied.And gonadotropin-releasing hormone whole brain precursor cGnRH, mGnRHstudied gene expression, not the cause of the initial demonstration ADSD macromarbled eel females produce significant induction of preliminary proved cpe cometogether and use hcg can marbled eel gonad ripening-related mechanisms, studiesshow: LH plays an important role in the marbled eel gonadal development process,with the expression of LH gonadal development has been increasing. The expressionlevels of FSH are all down, indicating that little role in the marbled eel gonadalmaturation process. Which GtHα upregulation also once again proved the importanceof gonadotropins during gonadal development in Anguilla, and raised from an earlyexpression was significantly GtHα common α subunit can be speculated that therecould be an early gonad development of thyroid hormone and FSH, LH presencehormone involved in the regulation. Quantitative results which brain cGnRH II andmGnRH two genes suggests that cGnRH II in the final expression of the mature stageseems to be higher, which initially described cGnRH II served a very important rolein the propagation of the latter process. And mGnRH throughout the induction processalso showed significantly higher for the indispensable role of gonadal development.4, in order to further explore the ADSD steroid injection marbled eel fish pro-generated effects to produce intrinsic role in how we generate cloned steroid active(cytochrome) gene fragment P45011β based on predecessors, and mRNAquantitative study was carried out. The entire breeding process their abundance is high, a preliminary indication for ADSD injection for Marbled P45011βH significantupregulation. But with marbled eel ovarian development is not directly related, butmay be related to certain correlation osmoregulation in migratory start and puberty.In summary, this paper practices and summarizes the key points of marbled eelartificial induction of gonad and spawning process, and prove cpe shared with hcg caneffectively induce marbled eel’s ovary and testis develope to maturation, andproblems arising in the course of ovaries to final maturation in depth discussion.fluorescence quantitative pcr research of genes related to gonad development:gonadotropin subunits (GtHα, LHβ, FSHβ), the precursor of gonadotropin-releasinghormone (cGnRH II, mGnRH), steroids such as generating enzyme gene mRNAP45011βconducted preliminary studies through the induction process of marbled eelartificial ripening process and the potential role of gene expression. Our studiesprovide an important basis for further understanding and of GnRH regulation andfunction in Japanese eel. |
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