| This experiment was conducted to investigate the effects of different calcium levels ofcontent on tibia quality, hematological biochemical indices and expression of critical signalfactors in Wnt/β-catenin signaling pathway in bone mass of piglets, and to estimate theinfluence of different calcium levels in dietary on osteoblasts of piglets.The experiment was a single factorial randomized design,60crossed pigs (Duroc×Landrace×Yorkshire,21.8±2.1kg) were randomly divided in6groups, groups，each groupinclud10animals. Pigs were fed with corn-soybean diets which were added with0.3%、0.45%、0.6%、0.75%、0.9%and1.05%calcium, respectively. The preliminary period included7days,the formal period included28days.The results are shown below：1There was remarkable effects of dietary calcium level onthe tibia quality of piglets (P<0.05), but no remarkable effect on feed conversion (P>0.05)，theeffects of different dietary calcium levels on calcium content of tibia、dry weight of defattedtibia、 ash content in defatted tibia and BMD of tibia were significant (P<0.05), and got thehighest values in0.75%group (P <0.05).2Alkaline phosphatase concentration in serum was decreased at first and increasedsubsequently with the dietary calcium increasing, and it got the minimum in0.75%dietarycalcium (P <0.05), osteocalcin concentration in serum was contrary to Alkaline phosphatase,and it reached the peak values in0.75%group (P <0.05).3The blood plasma contents of calcium were not significant differences among different dietary calcium contents (P<0.05),While the blood Parathyroid hormone and calcitonin were significantly effect by diet calcium content(P<0.05)．he blood Parathyroid hormone was decreased and the calcitonin was increased on0.90%and1.05%groups, and Vitamin D3levels in serum was not affected by diet calcium levels change (P>0.05).4Serum interleukini-2and interleukini-6contents were decreased at first and increasedsubsequently with the dietary calcium increasing, and they got the lowest values in0.75%group(P<0.05); the effects of different dietary calcium levels on BMD of tibia were significant(P<0.05), and got the highest values in0.75%group (P <0.05).5The differences of the calcium content in dietary could affect β-catenin (P<0.05), Withthe dietary calcium increasing, β-catenin mRNA expression in bone marrow was significantly decreased, reached the lowest values in0.75%group(P<0.05), soon afterwards, it increased.Wnt3a, Wnt10b expression in0.90%and1.05%groups were higher than others (P<0.05), buttheir lowest values appeared in0.75%and0.60%groups.Summarized them below that，the0.75%calcium for the piglets can increase the performance of tibia and the activity of osteoblasts. So,0.75%calcium is better for piglets of about20kg. |
PDF Full Text Request