Cloning And Expression Analysis Of Flowering Integrator Soc1in Brassica Juncea Coss.

Flowering is a critical period of plants from vegetative to reproductive growth transformation, It is an important part of plant growth and offspring, period can be adjusted and accelerate the breeding of varieties while early flower. Delay flowering can avoid premature in plants production. Therefore the time is important for seed and production. The main factors affecting flowering time, including temperature and light and other environmental factors and internal factors (plant growth status, developmental stage and genetic factors, etc.). Vernalization pathways、autonomous pathway、photoperiod and the gibberellin pathways are major flowering pathways. Although this flowering pathways have different gene regulatory networks, but it will eventually converge to a common flowering pathways integrator. Currently, a large number of flowering genes in Arabidopsis have been isolated, FLOWERING LOCUS C (FLC)、SUPPRESSOR OF CO OVEREXPRESSION1(SOC1)、FLOWERING LOCUS T (FT), gene FT and SOC1promote flowering, FLC is flowering suppressor gene. The Brassica juncea and Brassica oleracea are important Brassicaceae vegetables, Brassica juncea is breed vernalization type,but Brassica oleracea is body vernalization type.Therefore, the Research of flowering time regulation gene SOC1in Brassica juncea and Brassica oleracea, has a very important significance in the production and practice.The researching material is Brassica juncea and Brassica oleracea for, using conserved regions of flowering genes had been reporte to design a degenerate primers, Get a promote flowering genes SOCI in Brassica juncea and Brassica oleracea. SOCI in the two important Brassicaceae vegetables for sequence homology analysis, and use of the real-time quantitative RT-PCR, Understand the expression of gene SOCI in leaves and shoot apex of Brassica juncea in different environments flowering.1SOCI Cloning and AnalysisDesigning SOCI primers according to the published conservative region of Arabidopsis, SOCI gene Fragment have be cloned in Brassica juncea and Brassica oleracea. Brassica juncea SOCI gene contains625bp, encoding208amino acids, phylogenetic analysis showed kinship with Brassica oleracea SOCI (AFM77891.1). Brassica oleracea SOCI gene contains625bp, encoding209amino acids, Brassica campestris SOCI (AFH41826.1) is the closest relationship. by the two gene belong MIKC type MADS proteins, and Brassica juncea and Brassica oleracea SOCI encoded amino acid homology is88.99%. The comparison results of amino acid sequence show that a SOCI encoded protein in and Brassica olerace have MADS domain containing MADS MEF2-Like, is a member of MADS superfamily.2SOCI expression analysis in Autonomous pathwayAnalyzed the expression of flowering genes SOCI in Brassica juncea leaves and shoot tips in different pathways (autonomous pathway, photoperiod pathway and vernalization pathways) using real time fluorescence quantitative RT-PCR method. The results showed that, SOCI expression in Brassica juncea initial growth is very low, but with the plants growth, SOCI expression will increase. In autonomous pathway, SOCI expression of Brassica juncea will show a substantial increase at the first three periods, especially in the shoot apex, the first to reach the maximum amount of expression, the expression of SOCI of about7times than the second period, and then began to decrease.in the three period of leaves, the expression of SOCI about5times than the second period; in the forth period of leaves the expression rise seldomly, then begin to decline. This indicates that there is a complex gene regulatory networks regulating flowering in growth of Brassica juncea, this is purposes to reproduce.3SOCI expression analysis in Photoperiod pathwayIn the5period of Photoperiod pathway, in addition to the three period of leaves expression levels compared to the second period declined slightly, the expression of other periods have been rising. the maximum SOC1expression of leaves and shoot apex is the fifth period, except the third period, the SOC1of leaves is lower than the expression of shoot apex, SOC1expression of leaves in other period were higher than in the shoot apex. This suggests that long day promoted the expression of SOC1.4SOC1expression analysis in Vernalization pathwayAt low temperatures, shoot tips and leaves a very low expression levels in the first two periods, the expression in shoot apex beginning to rise in the third periods, while the expression in the fifth periods is rising obviously. The maximum amount of SOC1expression in leaves is the third periods, in this period, SOC1the expression level in leaves was significantly higher than the expression in shoot tips. This indicates that the a period low temperature inhibited the expression of flowering inhibiting factor.