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The Research On The Physiological And Biochemical Mechanism Of Oxygen Fertilizer Releasing The Soil Salt And Water Stress Of Taxodium Ascendens

Posted on:2015-10-05Degree:MasterType:Thesis
Country:ChinaCandidate:X XuFull Text:PDF
GTID:2283330428951836Subject:Biochemistry and Molecular Biology
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Coastal city and River City is the most densely populated and economic development area in our country. This is not only the main grain producing areas, but also the most vulnerable to the flood disaster area. Saline alkali land area accounts for7.26%of the total land area. People have to turn to the development of saline alkali soil to solve the problem with global arable land decreasing. There are currently about20%of agricultural land salinization in the world. Soil salinization has posed a serious threat to the ecological safety and the survival of mankind. Saline waterlogging stress has influence on plant almost all important life processes.It will cause the inhibition of normal growth and development of plants and even death. Study on a new type of oxygen fertilizer to release saline waterlogging stress is very meaningful.For example,it can help us recover production in the flood area. It can help us to develop saline alkali land which is temporarily unused. It can also help us to restore coastal vegetation to release saline waterlogging stress.This ecperiment chose Taxodium ascendens as experimental material. saline waterlogging was selected as the stress factor. Through artificial simulated saline waterlogging stress of Taxodium ascendens Seedlings. Experiment was completely randomized in design. With the physiological and biochemical method to study the mechanism of Taxodium ascendens suffered from saline waterlogging stress and the effect of oxygen fertilizer. We can determine the reasonable fertilization and fertilization methods by the change of physiological and biochemical indexes after we use oxygen fertilizer.At last, we can improve the pruduction process of oxygen fertilizer and ultimately the large-scale production. The main research harvests are as follows:1.Oxygen fertilizer tablets produced under dry conditions. Water will cause the loss of effective components of oxygen fertilizer, so producing oxygen fertilizer without any water can ensure the best oxygen fertilizer effect. The production of oxygen fertilizer took the application of machine equipment, so that it can reduce manual operation and improve the production efficiency. The various raw materials of oxygen fertilizer were screened out through the single factor experiment and cost factors.The raw materials for oxygen fertilizer must have the best effect and the highest price. Producing oxygen fertilizer tablets according to the ratio of1:1:1:10is the most reasonable.2.With the detection and tracking of40days, the pH of the solution was between7-8in the process of oxygen fertilizer releasing oxygen,which was very close to pure water.The oxygen fertilizer will not change the pH of the soil and cause any adverse effects on plant growth. The amount of dissolved oxygen in the oxygen fertilizer solution reached a maximum value at the twentieth day,and remained at a high level in the fortieth day. The dissolved oxygen curve of the oxygen fertilizer solution is more gentle than the curve of calcium peroxide solution. It indicates that oxygen fertilizer release oxygen lasting stability, and can provide enough oxygen for plant under long-term saline waterlogging stress.3. The maximum of SOD, POD activity and MDA content in leaves of Taxodium ascendens which under Saline waterlogging stress were3times,2.2times and3.5times of the Taxodium ascendens in normal control. Changes of each curve were very intense, indicated that Taxodium ascendens responsed to saline waterlogging stress strongly. Growth and metabolism of plants need the structure and function of cells remain relatively stable. The drastic changes is very bad for the normal growth of plants. The maximum of SOD、POD activity and MDA content in leaves of Taxodium ascendens were decreased by41%,25%and50%when each basin was added to the20g oxygen fertilizer. The maximum of SOD、POD activity and MDA content in leaves of Taxodium ascendens were decreased by49%,32.5%and54%when each basin was added to the40g oxygen fertilizer. The activity of SOD and POD got lower explained that the active oxygen free radicals produced less. The decrease of MDA content in plants indicated oxidative damage to the plant was reduced. These data suggested that the saline waterlogging stress of Taxodium ascendens effectively relieved and plants returned to normal growth after adding oxygen fertilizer.4. Compared to Taxodium ascendens which was added20g oxygen fertilizer, Variation curves of SOD、POD activity and MDA content in Taxodium ascendens leaves by adding40g oxygen fertilizer were more smooth and more close to the normal level.It indicated that40g oxygen fertilizer could better relieve the saline waterlogging stress of Taxodium ascendens and made it return to normal growth faster. So the more reasonable fertilizing amount of oxygen fertilizer is40g.
Keywords/Search Tags:oxygen fertilizer, saline waterlogging stress, Taxodium ascendens, physiological and biochemical indexes
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