Studies On Tissue Culture And Plant Regeneration Of Aquilaria Sinensis (Lour.) Spreng

It is a necessary guarantee to establish the regeneration system for shortening the breeding period aloes and maintaining the good traits.of Aquilaria sinensis (Lour.) Spreng. in vitro. Callus is a good material for the development of transgenic. Establishing the regeneration in vitro can provide the foundation for studies of genetic improvement for Aquilaria sinensis (Lour.) Spreng. in future.The objectives of the study were to examine tissue cultural development of different parts of explants of Aquilaria sinensis (Lour.) Spreng. to establish in vitro regeneration system.The embryonic callus, somatic embryos and the aseptic seedlings were induced successfully, which have established a foundation for future study of Aquilaria sinensis (Lour.) Spreng. genetic transformation.system and genetic engineering. Meanwhile, the study also support the spreading the technology of in vivo regeneration of Aquilaria sinensis (Lour.) Spreng. The main results of the study are as follows:1. The disinfection method system of different parts of explants was established up.(1) The best disinfecting for Aquilaria sinensis’s explants of leaves and stems is using0.1%HgCl2and eight-minute disinfection treatment, the highest survival rate was31.11%and65.56%.(2) The best disinfecting for Aquilaria sinensis’s explants of seeds is using0.2%HgCl2and eight-minute disinfection treatment, the highest survival rate was96.67%.2. Screened out the bud sprouting medium of Aquilaria sinensis’s stems.With the third to the fourth axillary bud stem section of the branch picked in March, the best bud sprouting medium of Aquilaria sinensis’stems in the concentration range designed is7/10MS+6-BA0.35mg/L+NAA0.01mg/L.The induction rate is93.33%.3. Screened out the bud proliferation medium of Aquilaria sinensis’s stems.The best bud proliferation medium of Aquilaria sinensis’stem in the concentration range designed is7/10MS+KT1.27mg/L+NAA0.2mg/L.The proliferation multiples is3.53.4. Screened out the callus induction and proliferation medium of Aquilaria sinensis’s leaves, stems, embryos (cotyledon) and roots.(1) The best callus induction and proliferation medium of Aquilaria sinensis’s leaves in the concentration range designed is MS+6-BA0.30mg/L+2,4-D0.34mg/L+CW10%(V/V). The rate of callus induction is44.83%.(2) The best callus induction and proliferation medium of Aquilaria sinensis’s stems in the concentration range designed is1/2MS+6-BA1.50mg/L+2,4-D1.50mg/L+CW 10%(V/V). The rate of callus induction is86.21%.And the better treatment for callus inducation of stem is peeling.(3) The best callus induction and proliferation medium of Aquilaria sinensis’s embryos (cotyledon) in the concentration range designed is MS+6-BA0.30mg/L+2,4-D0.30mg/L+CW10%(V/V). The rate of callus induction is93.10%.(4) The best callus induction and proliferation medium of Aquilaria sinensis’s roots in the concentration range designed is1/2MS+6-BA1.53mg/L+2,4-D1.40mg/L+CW10%(V/V).The rate of callus induction is20.00%.5. Screened out the callus differentiation medium of Aquilaria sinensis’s stems.The best callus differentiation medium of Aquilaria sinensis’stems in the concentration range designed is7/10MS+KT1.60mg/L+NAA0.20mg/L+CW20%(V/V).The differentiation rate is6.67%.6. Screened out the roost inducation medium of Aquilaria sinensis’s aseptic seedlings.The best roots inducation medium of Aquilaria sinensis’s aseptic seedlings in the concentration range designed is7/10MS+6-BA0.02mg/L+IBA1.23mg/L+CW25%(V/V).The rate of roots inducation is33.33%. The average root length is5cm.7. The tissue culture seedling transplanting technology system was explored preliminarily.Properly developed plantlets were transferred into the pots containing mixtures of sterilized vermiculite and fine river sand (ratios1:1) covered with a transparent plastic cups in order to achieve the role of hot insulation and moisture insulation.The seedlings were then transferred into the field when they were growing well.