Functional Characterization Of FoMsb2and FoHog1Gene In Fusarium Oxysporum F.sp.Cubense

Banana wilt pathogen Fusarium oxysporum f.sp.cubense,as a typical soil-borne diseases, which spread through the soil, it is difficult to control. Currently, the pathogenesis research of banana wilt disease was less, making its hard to targeted prevention. Functional studies on Fusarium oxysporum f.sp.cubense related genes can deepen our understanding of the pathogenic mechanisms, thereby providing new ideas for pathogen control.MAPK (Mitogen-Activated Protein Kinase) are a kind of widely exist protein kinase in eukaryotes involved in extracellular signaling, which are highly conserved in evolution pathway. In Fusarium oxysporum f.sp.cubense, FoMsb2gene encodes a transmembrane mucin, which mainly involved in the process of receiving and passing exogenous signal molecules downstream gene FoFmkl activity. Meanwhile FoHogl gene is also involved in pathogen response to exogenous processes such as osmotic pressure.We bioinformatics analysis FoMsb2and FoHogl genes.By constructing knockout vectors,using PEG-mediated protoplast transformation will be transformed into the knockout fragment pathogens obtained FoMsb2, FoMsb2+FoFmk1, FoHogl knocking genes.phenotype mutant analysis found that mutant AFoMsb2, AFoFmkl,△FoMsb2+△FoFmk1and AFoHogl colony growth rate slower than wild type,△FoHogl mutant only the1/4wild-type sporulation, and can not grow in high osmotic environment.Virulence determination was found that mutant△FoMsb2,△FoFmk1,△FoMsb2+△FoFmk1not penetrate cellophane and AFoFmkl,△FoMsb2+△FoFmk1gone on living tissue colonization,and AFoMsb2, AFoHogl fixed significantly reduced reproductive capacity. Further, the back mutation test found that the biological characteristics of the phenotype of the strain response is similar to the wild-type strain.In this study, We preliminarily elucidated functions identification of FoMsb2snd FoHogl,laid the foundation for further reveal the pathogenesis of banana wilt disease, the next step required for protein physicochemical properties and on the basis of mutual respect for research.