| Heilongjiang province is located in the northeast of Eurasia, belongs to the northern continental monsoon climate, heat resource is insufficient, in the rice booting to heading-flowering period, cold air activity is frequent, easily to cause disorder of chilling injury, to improve rice production was severely affected.Test east of farming428, loose japonica10,7dragon rice as the material, in booting stage three rice varieties respectively for different days of the cold water processing, the low temperature stress on different varieties of rice functional leaves and grain sucrose, fructose, soluble sugar content in the enzyme activity and function of SPS, SS, the effects of starch and starch components form the accumulation pattern, starch branching enzyme activity regulation of starch accumulation reveal booting stage different days of the cold water stress on rice panicle traits and the influence of yield and yield components and quality, provide theoretical basis for the research on cold tolerance in rice in heilongjiang province. The results show that:1. The function of three rice varieties leaf sucrose content in booting stage show unimodal curve changes after the cold stress, CK,3d,6d processing functions of sucrose content in the leaves at booting stage cold water stress peaked around28d after treatment, and9d,12d,15d after treatment is about35d maximum decline. Three varieties, the function leaf sucrose content is shown as:farmers428> rice7> pine japonica10dragon. Three rice varieties from7to35days after the full panicle, grain sucrose content have a downward trend after rising first, after the full panicle after21d around the flowers around10-20d d peak, after falling rapidly, both between the groups is to deal with a minimum of15d, three varieties, the grain sucrose content is shown as: farmers428>7> pine japonica rice10dragon.2. East428and dragon rice farmers7function fructose content in the leaves in the28d after cold water stress at the upper end and relatively loose japonica reach maximum10in stress after35d, conform to the "steady increase, the peak before and after collapse". Stress after7d to21d, three varieties of fructose content at the speed of slow growth,21d and28d three varieties of fructose content is growing at a faster pace, farmers428-35d and28d dragon rice7fructose content began to decline, pine japonica10fructose content continues to grow,35and42d after stress east428and dragon rice farmers7fructose content drop speed increase, stress after35d pine japonica10fructose content began to fall. Three rice varieties from7to35days after the full panicle, fructose content in grains were all showed a trend of decline after rising first, after14d around the flowers in full panicle after about10-20d d peak, after falling rapidly, three varieties, fructose content in functional leaves and grain are:farmers428> rice7> pine japonica10dragon.3. East428and dragon rice farmers7function leaf soluble sugar content in28d after cold water stress at the upper end and relatively loose japonica1035d peak after stress. Stress after7d to21d, three varieties of soluble sugar content in order to slow the speed of growth,21d,28d three varieties of soluble sugar content is growing at a faster pace, farmers428-35d and28d dragon rice soluble sugar content began to decline, pine japonica10soluble sugar content continues to grow,35and42d after stress east428and dragon rice farmers and soluble sugar content decreased speed increase, stress after35d pine japonica10began to fall. Three rice varieties from7to35days after the full panicle, soluble sugar content in the grains were all showed a trend of decline after rising first, after14d around the flowers in full panicle after about10-20d d peak, according to the rapid decline in the way after a slow first, after three varieties, soluble sugar content in functional leaves and grain are:farmers428>7> pine japonica rice10dragon.4. The three SPS activity in functional leaves of rice varieties were unimodal curve have been rising or falling,28d after the stress peak, after gradually decline. Performance is:the3d>> CK>6d9d>12d>15d. Within7to14d after cold water stress functions blades of SPS activity and activity changes between the amplitude difference is not obvious;14to28d, differences between SPS activity increases gradually. Function of SPS activity and sucrose, fructose content in leaf presents significant or extremely significant positive correlation. Three along with the growth period of rice varieties, functions are in surge before the SS activity in the leaves slowly increasing last fall.28d,7d after the cold stress-along with the growth period in accordance with the rapid growth of SS activity way, for the first28d-35d after stress SS activity growth rate began to fall, and the activity of CK>3d>6d>9d>12d>15d, stress after35d-42DSS activity began to decline.5. Three rice varieties with grouting starch and its components in grains were increase with the increase of the number of days.7d to21d after full panicle growth relatively quickly, after slow growth. Processing, with the increase of number of booting stage cold water stress decreases in the content of its its content is shown as:6d> CK>3d>9d>12k>15d, three varieties, the performance of starch and its components in grains were as follows:east farmers428> rice7> pine japonica10dragon.6. Three grain Q enzyme activity after grain full panicle rice varieties were unimodal curve, peak around21d after full panicle.14d and21d after full panicle, grain Q enzyme activity and amylose content was significantly correlated respectively. In addition to the7d,14d after full panicle, grain Q enzyme activity and negatively correlated with branched chain starch content, other times Q enzyme activity and amylopectin content in grains were very significant positive correlation, respectively. Full panicle after21d-35d, Q enzyme activity and total starch content in grain were significant or extremely significant positive correlation. 7. Booting stage cold water stress on rice appearance quality mainly reduced the influence of the grain and grain length of aspect ratio, increase the rate (chalky rice percentage of grain; The influence of the processing quality of rice mainly to reduce the rate of grain rate of brown rice, white rice, whole polished rice rate; The effect of nutrition and eating quality of rice are mainly reduced grain amylose content and gel consistency, increase the protein content of self-care and alkali value.8. Booting stage cold water stress on rice mainly spike length, the influence of primary branch number, secondary branch number, grain weight, and lower seed setting rate, made of rice panicle shape into effect of ear length, the influence of the secondary branch is opposite bigger, and the spike weight and a branch of a smaller effect; Factors on yield and its components mainly for the number of ears, grain Numbers per spike, setting rate, the lower, the spike grain number, seed setting rate is bigger. Three varieties of production performance is:the east farming rice7> pine japonica10428> dragon. |
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