Studies On Microorganisms Related To Algin Degradation In The Intestine Of Sea Urchin

The degradation of algin in the Macrocystis pyrifera cells is an essential process in the current algin industry as well as the development of Macrocystis pyrifera biological resources. Due to the high cost and low efficiency of physiochemical degradation of algin and its environmental damage effect, we focus on the microorganisms exist in the intestine of sea urchin which is a type of Macrocystis pyrifera consumer. We analyze the level of16SrDNA through PCR-DGGE, and we sort for the microorganisms using sodium alginate as the only carbon source in the intestine of sea urchin and explore the requirement for relevant enzyme synthesis via traditional microbiological centrifugation.One significant contribution we have made to the studies of Halomonas is that we have first identified a type of bacteria in the genus of Halomonas to which Sodium alginate is the only carbon source via microbiological as well as physio-biochemical assays and16SrDNA. We have also confirmed that the synthesis of alginate lyase mainly depends on pH and the concentration of Sodium alginate through analyzing the components of the fermentation media for the bacteria and orthogonal experimentation.