Genetic Mapping Of Fruit Sugar Related Genes In Melon

Melon (Cucumis melon l.) is one of the important horticultural crops worldwide, whichis famous for its fragrant, crisp juice and sweet flavor. Sugar content is considered as one ofmajor traits of melon quality measurement, and it is the basis component of fruit quality andflavor as well as vitamin, aromatic and pigment synthesis. In addition, sugar has providedpenetration impetus function for fruit cell expands.A recombinant Inbred Line (Recombinant Inbred Lines, RIL) F4which was constructedby melon “Furong”(high sugar content) and “CaiguaⅢ”(low sugar content) was used to testthe effect of AMAMIR TD-2010C portable fruit sugar instrument. The test was carried outnondestructive in field. We selected SSR molecular markers which linked with melonsugar-related genes. In this study, several melon sugar-related genes were preliminarilyestablished in melon genetic map.Sugar content of melon was tested respectively with AMAMIR TD–2010C portablefruit sugar instrument and handheld refractive sugar. The linear correlation of the twomethods was analyzed. Results showed that the nondestructive test instrument and handheldrefractometer had correlation and the correlation coefficient (R) was0.629. By detectinganother two different commercial melons, correction parameters and correlations were foundto be different. These results indicated that the sugar content detected by AMAMIR TD–2010C portable would be affected by melon varieties. In addition, variance analysis resultsshowed that both the environment light intensity and melon peel color measurement couldinfluenced data of total soluble solids. Among these two factors, the environment lightintensity was positively related. Data collected by the nondestructive instrument in thedark-peel area were higher than that of the light. Therefore, we consider that thenon-destructive sugar detector is easily affected by various and environmental factors. Thesefactors should be controlled during the nondestructive sugar detector used to test sugarcontent in the field. 396pairs of SSR markers covered12Linkage Groups and fruit sugar contact QTL(Quantitative Trait loci) were studied in RILs. The results indicated that one melonsugar-related QTL (CMGT10-ECM223) were located in LGII. This study studied the melonsugar-related gene loci which lay a foundation to further determine the location and sequenceof melon sugar gene.